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QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins

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Autor(es):
Soratto, Tatiany Aparecida Teixeira ; Curtolo, Maiara ; Marengo, Samanta ; Dezotti, Ana Lucia ; Lima, Romulo Pedro Macedo ; Gazaffi, Rodrigo ; Machado, Marcos Antonio ; Cristofani-Yaly, Mariangela
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: ROPICAL PLANT PATHOLOG; v. 45, n. 6, p. 20-pg., 2020-05-29.
Resumo

Huanglongbing (HLB) is a severe disease of citrus caused by the bacteriumCandidatusLiberibacter. In America, the most common species isCandidatusLiberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization betweenCitrus sunkiHort. Ex Tan. andPoncirus trifoliata(L.) Raf. cv. Rubidoux. The bacterial concentration was higher inC. sunkithan inP. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps ofC. sunkiandP. trifoliatacv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) betweenC. sunkiandP. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in theC. sunkimap and 17 QTL and 40 eQTL in theP. trifoliatamap. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. (AU)

Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
Beneficiário:Marcos Antonio Machado
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/22133-0 - Edição de genoma visando à validação de eQTLs associados à tolerância a Candidatus Liberibacter asiaticus em citrandarins (Citrus sunki x Poncirus trifoliata)
Beneficiário:Maiara Curtolo
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/00133-4 - Ensaios com citrandarins como porta-enxertos para as laranjas Pêra e Valência e a lima ácida Tahiti
Beneficiário:Mariângela Cristofani-Yaly
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/18605-0 - Obtenção e avaliação de novas variedades copas e porta-enxertos para citricultura de mesa
Beneficiário:Mariângela Cristofani-Yaly
Modalidade de apoio: Auxílio à Pesquisa - Temático