Micro-MsK: a tomato genotype with miniature size, short life cycle, and improved in vitro shoot regeneration

Autor(es)

Lima, Joni E. ; Carvalho, Rogério F. ; Tulmann Neto, Augusto ; Figueira, Antonio ; Peres, Lázaro Eustáquio Pereira ^[5]

• Tipo de documento: Artigo Científico
• Fonte: Plant Science; v. 167, n. 4, p. 753-757, 2004.
• Área do conhecimento: Ciências Agrárias - Agronomia
• Assunto(s): Fisiologia vegetal Análise de sequência de DNA Hibridização genética Tomate Lycopersicon esculentum

Resumo

The in vitro regeneration ability of tomato (Lycopersicon esculentum Mill.) remains the main limiting factor for efficient genetic transformation. However, competence for in vitro regeneration can be transferred from wild Lycopersicon species to the cultivated tomato. The high regeneration capacity presented by the MsK genotype, derived from L. peruvianum, was attributed to the dominant loci Rg-1 and Rg-2. In the present work, we have transferred the high organogenetic competence from the MsK genotype to the cultivar Micro-Tom, which presents miniature size (8-cm tall) and a rapid life cycle (75 days from seed to fruit ripening). We obtained stable and non-segregating F6 Micro-MsK plants, which were expressively superior to Micro-Tom in regeneration capacity, when hypocotyls but not cotyledons were used as explants. In addition to the regeneration ability, the obtained genotype presented a delayed leaf senescence and a reduced apical dominance. The development of Micro-MsK genotype will enable the application of insertional mutagenesis in functional genomics of tomato. (AU)

• Processo FAPESP: 02/00329-8 - Estudo das bases genéticas e bioquímicas da competência para regeneração in vitro utilizando micro tomateiro (Lycopersicon esculentum cv Micro-Tom) como modelo experimental
• Beneficiário: Lázaro Eustaquio Pereira Peres
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores