Alternative Splicing in Trichophyton rubrum Occurs in Efflux Pump Transcripts in Response to Antifungal Drugs

Autor(es)

Lopes, Marcos E. R. ; Bitencourt, Tamires A. ; Sanches, Pablo R. ; Martins, Maira P. ; Oliveira, Vanderci M. ; Rossi, Antonio ; Martinez-Rossi, Nilce M.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 8, n. 8, p. 12-pg., 2022-08-01.

Resumo

Dermatophytes are challenging to treat because they have developed many strategies to neutralize the stress triggered by antifungals. Drug tolerance is achieved by mechanisms such as drug efflux and biofilm formation, and cellular efflux is a consequence of the synergistic and compensatory regulation of efflux pumps. Alternative splicing (AS) has also been considered as a mechanism that enhances fungal adaptive responses. We used RNA-seq data from the dermatophyte Trichophyton rubrum exposed to undecanoic acid (UDA) to search for and validate AS in genes encoding efflux pumps. The magnitude of this phenomenon was evaluated using UDA and other antifungals (caspofungin, itraconazole, and terbinafine) in planktonic and biofilm cultures. In addition to the conventional isoforms, the efflux pump encoded by TERG_04309 presented two intron-retained isoforms. Biofilms trigger the simultaneous production of at least two isoforms. The intron-retained isoforms showed short lengths and topologically different organization. Furthermore, we identified the putative interaction of efflux pumps (TERG_04309 and TERG_04224). Co-expression of these genes suggests a synergistic action in antifungal resistance. Our data provide new insights into drug tolerance related to differential isoform usage and the co-expression of stress-responsive genes, which may lead to higher antifungal resistance, mainly in biofilms. (AU)

• Processo FAPESP: 18/11319-1 - Fatores de transcrição e sinalização celular.
• Beneficiário: Maíra Pompeu Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/23435-8 - Mecanismos moleculares envolvidos na resistência e na resposta adaptativa a inibidores fúngicos
• Beneficiário: Tamires Aparecida Bitencourt
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/22596-9 - Mecanismos moleculares associados à patogenicidade e resistência em fungos: estratégias para o tratamento de dermatofitoses
• Beneficiário: Nilce Maria Martinez-Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Temático