IL10/AMPK pathway was associated with the hippocampal anti-inflammatory response to high-sugar and high-fat diet withdrawal

Autor(es)

Casagrande, Breno Picin ; Pisani, Luciana Pellegrini ; Estadella, Debora

• Tipo de documento: Artigo Científico
• Fonte: Inflammation Research; v. 71, n. 10-11, p. 10-pg., 2022-09-09.

Resumo

Objective The present experimental study aimed to evaluate the effect of consuming an obesogenic diet (OD) on serum and hippocampal inflammation and proteins related to energy metabolism, alongside, we evaluated how the same parameters responded to an OD withdrawal. Subjects Thirty male 60-days-old Wistar rats were used. Methods The control group (n = 10) was fed the control diet across the whole experiment. The remaining animals were fed a high-sugar/high-fat (HSHF) diet for 30 days (n = 20) and half of them were placed on the control diet for 48 h (n = 10) afterwards. Results OD intake decreased hippocampal AMPK phosphorylation, although, it did not increase serum inflammation and only increased hippocampal pNF kappa Bp65 levels without any increase in the cytokines assessed. Moreover, OD withdrawal led to higher inflammatory markers in the serum and hippocampus and higher hippocampal AMPK phosphorylation. The mediation models applied suggested that the effect of OD withdrawal on hippocampal inflammation was driven by serum inflammation, which activated the hippocampal IL10/AMPK anti-inflammatory pathway as a response. Conclusion Our analyses suggest that OD withdrawal increases serum inflammation with hippocampal consequent inflammatory alterations. Despite the general assumption that improving diet improves health, this may not be immediate. (AU)

• Processo FAPESP: 19/22511-3 - Estratégia para minimizar os efeitos adversos da substituição de dieta obesogênica por dieta padrão sobre parâmetros comportamentais e neuroendócrinos em um modelo animal
• Beneficiário: Breno Picin Casagrande
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/25420-3 - Efeitos do consumo de dieta hiperlipídica palatável e exercício sobre parâmetros inflamatórios centrais e periféricos em ratas e sua relação com comportamentos do tipo ansiedade e depressão
• Beneficiário: Debora Estadella
• Modalidade de apoio: Auxílio à Pesquisa - Regular