Quantum-Dot-Based Iron Oxide Nanoparticles Activate the NLRP3 Inflammasome in Murine Bone Marrow-Derived Dendritic Cells

Autor(es)

de Melo, Fernando Menegatti ; Kawasaki, Karine ; Sellani, Tarciso Almeida ; Bonifacio, Bruno Souza ; Mortara, Renato Arruda ; Toma, Henrique Eisi ; de Melo, Filipe Menegatti ; Rodrigues, Elaine Guadelupe

• Tipo de documento: Artigo Científico
• Fonte: NANOMATERIALS; v. 12, n. 18, p. 18-pg., 2022-09-01.

Resumo

Inflammasomes are cytosolic complexes composed of a Nod-like receptor, NLR, the adaptor protein, ASC, and a proteolytic enzyme, caspase-1. Inflammasome activation leads to caspase-1 activation and promotes functional maturation of IL-1 beta and IL-18, two prototypical inflammatory cytokines. Besides, inflammasome activation leads to pyroptosis, an inflammatory type of cell death. Inflammasomes are vital for the host to cope with foreign pathogens or tissue damage. Herein, we show that quantum-dot-based iron oxide nanoparticles, MNP@QD, trigger NLRP3 inflammasome activation and subsequent release of proinflammatory interleukin IL-1 beta by murine bone marrow-derived dendritic cells (BMDCs). This activation is more pronounced if these cells endocytose the nanoparticles before receiving inflammatory stimulation. MNP@QD was characterized by using imaging techniques like transmission electron microscopy, fluorescence microscopy, and atomic force microscopy, as well as physical and spectroscopical techniques such as fluorescence spectroscopy and powder diffraction. These findings may open the possibility of using the composite MNP@QD as both an imaging and a therapeutic tool. (AU)

• Processo FAPESP: 18/21489-1 - Nanotecnologia supramolecular: design, materiais e dispositivos
• Beneficiário: Henrique Eisi Toma
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/08180-6 - Estudo da regulação dos inflamassomos exercida pelo Sistema Nervoso Simpático (SNS)
• Beneficiário: Filipe Menegatti de Melo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/04948-5 - Estudo da regulação dos inflamassomos exercida pelo sistema nervoso simpático (sns)
• Beneficiário: Filipe Menegatti de Melo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado