Reactivity of DENV-positive sera against recombinant envelope proteins produced in bacteria and eukaryotic cells

Autor(es)

Souza, Higo Fernando Santos ; Zaneti, Arthur Baruel ; Almeida, Bianca da Silva ; Martinho, Jessica Amaral ; Yamamoto, Marcio Massao ; Rosa, Daniela Santoro ; Slhessarenko, Renata Denzegrini ; Boscardin, Silvia Beatriz

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGIC RESEARCH; v. 71, n. 1, p. 12-pg., 2022-10-03.

Resumo

Dengue is a mosquito-borne disease endemic in many tropical and subtropical countries. It is caused by the dengue virus (DENV) that can be classified into 4 different serotypes (DENV-1-4). Early diagnosis and management can reduce morbidity and mortality rates of severe forms of the disease, as well as decrease the risk of larger outbreaks. Hiperendemicity in some regions of the world and the possibility that some people develop a more severe form of disease after a secondary infection caused by antibody-dependent enhancement justify the need to understand more thoroughly the antibody response induced against the virus. Here, we successfully produced a recombinant DENV-2 envelope (E) protein and its domains (EDI/II and EDIII) in two distinct expression systems: the Drosophila S2 insect cell system and the BL21 (DE3) pLySs bacterial system. We then evaluated the reactivity of sera from patients previously infected with DENV to each recombinant protein and to each domain separately. Our results show that the E protein produced in Drosophila S2 cells is recognized more frequently than the protein produced in bacteria. However, the recognition of E protein produced in bacteria correlates better with the DENV-2 sera neutralization capacity. The results described here emphasize the differences observed when antigens produced in bacteria or eukaryotic cells are used and may be useful to gain more insight into the humoral immune responses induced by dengue infection. (AU)

• Processo FAPESP: 17/26342-6 - Análise da resposta humoral induzida pela infecção com o vírus Zika utilizando anticorpos monoclonais produzidos a partir de linfócitos B isolados de pacientes infectados.
• Beneficiário: Bianca da Silva Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/07142-9 - Influência das Vias de Sinalização de STAT1, STAT3, STAT5 e STAT6 em Células Dendríticas Convencionais na Instrução da Resposta de Células T Auxiliares
• Beneficiário: Silvia Beatriz Boscardin
• Modalidade de apoio: Auxílio à Pesquisa - Regular