Antisense Transcription in Plants: A Systematic Review and an Update on cis-NATs of Sugarcane

Autor(es)

Santini, Luciane ; Yoshida, Leonardo ; de Oliveira, Kaique Dias ; Lembke, Carolina Gimiliani ; Diniz, Augusto Lima ; Cantelli, Geraldo Cesar ; Nishiyama-Junior, Milton Yutaka ; Souza, Glaucia Mendes

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 19, p. 21-pg., 2022-10-01.

Resumo

Initially, natural antisense transcripts (NATs, natRNAs, or asRNAs) were considered repressors; however, their functions in gene regulation are diverse. Positive, negative, or neutral correlations to the cognate gene expression have been noted. Although the first studies were published about 50 years ago, there is still much to be investigated regarding antisense transcripts in plants. A systematic review of scientific publications available in the Web of Science databases was conducted to contextualize how the studying of antisense transcripts has been addressed. Studies were classified considering three categories: "Natural antisense" (208), artificial antisense used in "Genetic Engineering" (797), or "Natural antisense and Genetic Engineering"-related publications (96). A similar string was used for a systematic search in the NCBI Gene database. Of the 1132 antisense sequences found for plants, only 0.8% were cited in PubMed and had antisense information confirmed. This value was the lowest when compared to fungi (2.9%), bacteria (2.3%), and mice (54.1%). Finally, we present an update for the cis-NATs identified in Saccharum spp. Of the 1413 antisense transcripts found in different experiments, 25 showed concordant expressions, 22 were discordant, 1264 did not correlate with the cognate genes, and 102 presented variable results depending on the experiment. (AU)

• Processo FAPESP: 08/52146-0 - Sugarcane signaling and regulatory networks
• Beneficiário: Glaucia Mendes Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 20/07851-0 - Estudo da expressão gênica de genes relacionados ao desenvolvimento e acúmulo de biomassa em cana-de-açúcar
• Beneficiário: Kaique Dias de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/10876-4 - Ferramentas de bioinformática para integração de dados na plataforma SUCEST-FUN
• Beneficiário: Geraldo Cesar Cantelli
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 14/50921-8 - Redes regulatórias e sinalização associadas à cana energia
• Beneficiário: Glaucia Mendes Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 18/09867-0 - Obtenção da cana energia por transgenia
• Beneficiário: Luciane Santini Gazaffi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/02270-6 - Avaliação do transcriptoma de progênies contrastantes para o acúmulo de biomassa e identificação de redes de regulação
• Beneficiário: Augusto Lima Diniz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado