Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel

Autor(es)

P.P. Apolinário ^[1] ; F.C. Zanchetta ^[2] ; J.S.C. Breder ^[3] ; G. Adams ^[4] ; S.R. Consonni ^[5] ; R. Gillis ^[6] ; M.J.A. Saad ^[7] ; M.H.M. Lima ^[8]

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas. Colégio Técnico de Campinas - Brasil

^[2] Universidade Estadual de Campinas. Faculdade de Enfermagem - Brasil

^[3] Universidade Estadual de Campinas. Faculdade de Enfermagem - Brasil

^[4] University of Nottingham. Faculty of Medicine and Health Science - Ucrânia

^[5] Universidade Estadual de Campinas. Insituto de Biologia - Brasil

^[6] Sheffield Hallam University. Department of Service Sector Management - Ucrânia

^[7] Universidade Estadual de Campinas. Faculdade de Ciências Médicas - Brasil

^[8] Universidade Estadual de Campinas. Faculdade de Enfermagem - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 56, 2023-05-15.

Resumo

Diabetes mellitus is associated with impaired wound healing. The topical use of insulin is a promising therapy because it may favor all phases of the wound healing process. This study aimed to investigate the therapeutic outcomes of insulin gel in wounds of hyperglycemic mice. After diabetes induction, a 1-cm2 full-thickness wound was created on each animal's dorsum. The lesions were treated daily for 14 days with insulin gel (insulin group) or vehicle gel without insulin (vehicle group). Tissue samples were extracted on days 4, 7, 10, and 14 after the creation of the lesion. The samples were analyzed with hematoxylin/eosin and Sirius red staining, immunohistochemistry, Bio-Plex immunoassays, and western blotting. Insulin gel favored re-epithelialization at day 10 and increased the organization and deposition of collagen. Additionally, it modulated the expression of cytokines (interleukin (IL)-4 and IL-10) and increased the expression of arginase I, VEGF receptor 1, and VEGF on day 10. Activation of the insulin signaling pathway occurred via IRβ, IRS1, and IKK on day 10 and activation of Akt and IRS1 on day 14. These results suggested that insulin gel improved wound healing in hyperglycemic mice by modulating the expression of inflammatory factors, growth factors, and proteins of the insulin signaling pathway. (AU)

• Processo FAPESP: 14/50907-5 - INCT 2014 - de obesidade e diabetes
• Beneficiário: Mario Jose Abdalla Saad
• Modalidade de apoio: Auxílio à Pesquisa - Temático