Final irrigation protocols can be used to promote stable long-term bond strength of AH Plus to dentin

Autor(es)

Talita TARTARI ; Caroline WICHNIESKI ; Renato Menezes SILVA ^[3] ; Ariadne LETRA ; Marco Antonio Hungaro DUARTE ^[5] ; Clovis Monteiro BRAMANTE ^[6]

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 31, 2023-05-26.

Resumo

Abstract Irrigation solutions might affect dentin surface characteristics and, consequently, endodontic sealers adhesion. Objective This study analyzed the effect of different final irrigation protocols on push-out bond strength (BS) of AH Plus to dentin seven days and 20 months after obturation. Scanning electron micrographs were obtained from the dentin surface of one sample/group after final irrigation. Methodology Canals of bovine incisors were instrumented and received final irrigation with (n=21): G1 – 2.5% sodium hypochlorite (NaOCl) + distilled water; G2 – 2.5% NaOCl + 17% EDTA; G3 – 2.5% NaOCl + 17% EDTA + 2.5% NaOCl; G4 – 2.5% NaOCl + 17% EDTA + 2% chlorhexidine (CHX); G5 – mixture 5% NaOCl + 18% etidronate (HEDP); and G6 – mixture 5% NaOCl + 10% tetrasodium EDTA (Na4EDTA). After irrigation, one root/group was split and images were obtained by scanning electron microscopy (SEM). The other 20 roots/group were filled with only AH Plus sealer. Three slices/root were used for push-out assessment seven days and 20 months after obturation. One-way analysis of variance and Tukey (α<0.05) were used to compare the results among experimental groups, and unpaired t-test (α<0.05) was used to compare the results of the same group over time. Results The photomicrographs showed that, excepting G1, all groups completely removed the smear layer from the samples. In G2 and G4, the opening of the dentin tubules enlarged. In G3, erosion was observed in the peritubular and intertubular dentin. Values of the BS in the seven days were G2=G3=G4=G5>G6=G1 and in the 20 months were G3=G5>G6=G4>G1=G2. G3, G5, and G6 presented values of BS in 20 months similar to the values of seven days (P>0.05). Conclusions The final irrigation protocols tested produced dentin surfaces with different characteristics. Only G3 and G5 presented high BS values that were stable over time. (AU)

• Processo FAPESP: 15/09958-8 - Efeito de diferentes protocolos de irrigação na adesão e diferenciação de células-tronco da papila apical
• Beneficiário: Talita Tartari
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/22364-5 - Efeitos do uso de misturas de hipoclorito de sódio com diferentes agentes quelantes associadas ou não a agitação ultrassônica passiva na limpeza dentinária e extrusão de debris, na viabilidade celular, inflamação tecidual e desmetalização de biofilmes.
• Beneficiário: Marco Antonio Hungaro Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/12690-5 - Efeitos das misturas de hipoclorito de sódio com etidronato e EDTA tetrassódico alcalino associadas ou não a métodos de agitação na estrutura e limpeza dentinárias, extrusão de debris, na viabilidade celular e inflamação tecidual e em biofilmes microbiano
• Beneficiário: Talita Tartari
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/19789-3 - Efeitos de diferentes regimes de irrigação nas propriedades físico-químicas da dentina e consequências das alterações na adesão de microrganismos e do cimento endodôntico AH Plus
• Beneficiário: Talita Tartari
• Modalidade de apoio: Bolsas no Brasil - Doutorado