Final irrigation protocols can be used to promote stable long-term bond strength of AH Plus to dentin

Author(s)

Talita TARTARI ; Caroline WICHNIESKI ; Renato Menezes SILVA ^[3] ; Ariadne LETRA ; Marco Antonio Hungaro DUARTE ^[5] ; Clovis Monteiro BRAMANTE ^[6]

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 31, 2023-05-26.

Abstract

Abstract Irrigation solutions might affect dentin surface characteristics and, consequently, endodontic sealers adhesion. Objective This study analyzed the effect of different final irrigation protocols on push-out bond strength (BS) of AH Plus to dentin seven days and 20 months after obturation. Scanning electron micrographs were obtained from the dentin surface of one sample/group after final irrigation. Methodology Canals of bovine incisors were instrumented and received final irrigation with (n=21): G1 – 2.5% sodium hypochlorite (NaOCl) + distilled water; G2 – 2.5% NaOCl + 17% EDTA; G3 – 2.5% NaOCl + 17% EDTA + 2.5% NaOCl; G4 – 2.5% NaOCl + 17% EDTA + 2% chlorhexidine (CHX); G5 – mixture 5% NaOCl + 18% etidronate (HEDP); and G6 – mixture 5% NaOCl + 10% tetrasodium EDTA (Na4EDTA). After irrigation, one root/group was split and images were obtained by scanning electron microscopy (SEM). The other 20 roots/group were filled with only AH Plus sealer. Three slices/root were used for push-out assessment seven days and 20 months after obturation. One-way analysis of variance and Tukey (α<0.05) were used to compare the results among experimental groups, and unpaired t-test (α<0.05) was used to compare the results of the same group over time. Results The photomicrographs showed that, excepting G1, all groups completely removed the smear layer from the samples. In G2 and G4, the opening of the dentin tubules enlarged. In G3, erosion was observed in the peritubular and intertubular dentin. Values of the BS in the seven days were G2=G3=G4=G5>G6=G1 and in the 20 months were G3=G5>G6=G4>G1=G2. G3, G5, and G6 presented values of BS in 20 months similar to the values of seven days (P>0.05). Conclusions The final irrigation protocols tested produced dentin surfaces with different characteristics. Only G3 and G5 presented high BS values that were stable over time. (AU)

• FAPESP's process: 15/09958-8 - Effect of different irrigation protocols on the attachment and differentiation of stem cells from the apical papilla
• Grantee: Talita Tartari
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/22364-5 - Effects of using sodium hypochlorite mixtures with different chelating agents associated with or without passive ultrasonic agitation on dentin cleaning and extrusion of debris, on cell viability, tissue inflammation and demetalization of biofilms.
• Grantee: Marco Antonio Hungaro Duarte
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/12690-5 - Effects of mixtures of sodium hypochlorite with etidronate and alkaline tetrasodium EDTA associated or not with agitation methods on dentin structure and cleaning, debris extrusion, cell viability and tissue inflammation, and microbial biofilms
• Grantee: Talita Tartari
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/19789-3 - Effects of different irrigation regimes in physico-chemical properties of dentin and consequences of changesin the adhesion of microorganisms and AH Plus sealer
• Grantee: Talita Tartari
• Support Opportunities: Scholarships in Brazil - Doctorate