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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of anaerobic or/and microaerophilic atmosphere on microcosm biofilm formation and tooth demineralization

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Autor(es):
Aline Silva BRAGA [1] ; KIM Rafaela Ricci [2] ; Ana Carolina MAGALHÃES [3]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas - Brasil
[2] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas - Brasil
[3] Universidade de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciências Biológicas - Brasil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Applied Oral Science; v. 31, 2023-06-02.
Resumo

Abstract Microcosm biofilms can reproduce the complexity of a dental biofilm. However, different forms of cultivation have been used. The impact of the culture atmosphere on the development of microcosm biofilms and their potential to cause tooth demineralization has not yet been deeply studied. Objective This study analyzes the effects of three experimental cultivation models (microaerophile vs. anaerobiosis vs. experimental mixed) on the colony-forming units (CFU) of the cariogenic microorganisms and tooth demineralization. Methodology 90 bovine enamel and 90 dentin specimens were distributed into different atmospheres: 1) microaerophilia (5 days, 5% CO2); 2) anaerobiosis (5 days, jar); 3) mixed (2 days microaerophilia and 3 days anaerobiosis), which were treated with 0.12% chlorhexidine (positive control – CHX) or Phosphate-Buffered Saline (negative control – PBS) (n=15). Human saliva and McBain’s saliva containing 0.2% sucrose were used for microcosm biofilm formation, for 5 days. From the second day to the end of the experiment, the specimens were treated with CHX or PBS (1x1 min/day). Colony-forming units (CFU) were counted, and tooth demineralization was analyzed using transverse microradiography (TMR). Data were subjected to two-way ANOVA and Tukey’s or Sidak’s test (p<0.05). Results CHX was able to reduce total microorganism’s CFU compared to PBS (differences of 0.3–1.48 log10 CFU/mL), except for anaerobiosis and microaerophilia in enamel and dentin biofilm, respectively. In the case of dentin, no effect of CHX on Lactobacillus spp. was observed. CHX significantly reduced enamel demineralization compared to PBS (78% and 22% reductions for enamel and dentin, respectively). Enamel mineral loss did not differ when compared with the other atmospheres; however, the enamel lesion depth was greater under anaerobiosis. Dentin mineral loss was lower under anaerobiosis when compared with the other atmospheres. Conclusion The type of atmosphere has, in general, little influence on the cariogenic ability of the microcosm biofilm. (AU)

Processo FAPESP: 19/01730-9 - Efeito do adoçante Stevia sobre o desenvolvimento da cárie dentária em esmalte sob um modelo de biofilme microcosmo
Beneficiário:Rafaela Ricci Kim
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/17249-2 - Avaliação do efeito de diferentes extratos naturais experimentais e comerciais, na forma de dentifrícios e enxaguatórios bucais, sobre a viabilidade e atividade de biofilme microcosmo e sobre a prevenção da desmineralização do esmalte in vitro
Beneficiário:Aline Silva Braga
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 19/21797-0 - Efeito de formulações de saliva artificial e aplicação de fluoretos sobre o desenvolvimento de cárie radicular em modelo de biofilme microcosmo simulando pacientes submetidos à radiação de cabeça e pescoço
Beneficiário:Ana Carolina Magalhães
Modalidade de apoio: Auxílio à Pesquisa - Regular