Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer

Autor(es)

Bandeira Ferreira, Luiz Alberto ; Garcia-Fossa, Fernanda ; Radaic, Allan ; Duran, Nelson ; Favaro, Wagner Jose ; de Jesus, Marcelo Bispo

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS; v. 151, p. 9-pg., 2020-06-01.

Resumo

Bladder cancer is the fifth most common disease in the United States, and the treatment and alternatives for patients have not changed in the last decades. Silver nanoparticles (AgNP) have been used in the treatment of various cancer, mainly because of the antineoplastic activity; however, their use and the molecular mechanisms towards bladder cancer still unexplored. Therefore, this work aims to evaluate the in vitro and in vivo antitumoral mechanisms of biogenic silver nanoparticles synthesized from Fusarium sp. First, AgNP showed cytotoxicity in a dose- and time-response relationship and detailed analysis demonstrated the induction of cell death via apoptosis, also inhibiting cell migration and proliferation in bladder carcinoma cell line 5637. Next, it was evaluated the antitumoral activity of AgNP against non-muscle invasive bladder cancer (NMIBC). Bladder cancer was chemically induced with N-methyl-N-nitrosourea (MNU) on C57BL/6JUnib female mice and treated by intravesical route with AgNP concentrations of 0.5, 0.2, and 0.05 mg/mL. Finally, treatment with AgNP (0.05 mg/mL) led to 57.13% of tumor regression, with 14.28% of the animals showing normal urothelium, and 42.85% showing flat hyperplasia, considered to be a benign lesion. Overall, these findings demonstrated that AgNP might be a cost-effective alternative and promising candidate for the treatment of bladder cancer. (AU)

• Processo FAPESP: 15/06134-4 - EMU concedido no processo 2014/03002-7: leitor multi-modo de imageamento celular (cell imaging multi-mode reader)
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 14/03002-7 - Internalização e tráfego intracelular de nanopartículas: atividade biológica e perfil nanotoxicológico
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores