| Texto completo | |
| Autor(es): |
Silva, Rafael Marques da
;
Saladino, Giovanna Christe dos Reis
;
Leonardo, Diego Antonio
;
Pereira, Humberto D'Muniz
;
Araujo, Ana Paula Ulian
;
Garratt, Richard Charles
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Structural Biology; v. 215, n. 3, p. 13-pg., 2023-06-16. |
| Resumo | |
Septins, often described as the fourth component of the cytoskeleton, are structural proteins found in a vast variety of living beings. They are related to small GTPases and thus, generally, present GTPase activity which may play an important (although incompletely understood) role in their organization and function. Septins polymerize into long non-polar filaments, in which each subunit interacts with two others by alternating interfaces, NC and G. In Saccharomyces cerevisiae four septins are organized in the following manner, [Cdc11Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Cdc11]n in order to form filaments. Although septins were originally discovered in yeast and much is known regarding their biochemistry and function, only limited structural information about them is currently available. Here we present crystal structures of Cdc3/Cdc10 which provide the first view of the physiological interfaces formed by yeast septins. The G-interface has properties which place it in between that formed by SEPT2/SEPT6 and SEPT7/SEPT3 in human filaments. Switch I from Cdc10 contributes significantly to the interface, whereas in Cdc3 it is largely disorded. However, the significant negative charge density of the latter suggests it may have a unique role. At the NC-interface, we describe an elegant means by which the sidechain of a glutamine from helix & alpha;0 imitates a peptide group in order to retain hydrogen-bond continuity at the kink between helices & alpha;5 and & alpha;6 in the neighbouring subunit, thereby justifying the conservation of the helical distortion. Its absence from Cdc11, along with this structure's other unusual features are critically discussed by comparison with Cdc3 and Cdc10. (AU) | |
| Processo FAPESP: | 21/08158-9 - Estudos cristalográficos de septinas, seus domínios e seus complexos |
| Beneficiário: | Diego Antonio Leonardo Cabrejos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 20/02897-1 - Filamentos de septinas: estrutura, polimerização e atuação em patologias |
| Beneficiário: | Richard Charles Garratt |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 22/00125-7 - A biologia estrutural de septinas de organismos modelo |
| Beneficiário: | Giovanna Christe dos Reis Saladino |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 14/15546-1 - Septinas: estudos comparativos visando correlacionar estrutura e função |
| Beneficiário: | Richard Charles Garratt |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/09687-2 - Caracterização estrutural das septinas de levedura (Saccharomyces cerevisiae) Cdc3, Cdc10, Cdc11 e Cdc12 |
| Beneficiário: | Rafael Marques da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |