Xylose Isomerase Depletion Enhances Virulence of Xanthomonas citri subsp. citri in Citrus aurantifolia

Autor(es)

Alexandrino, Andre Vessoni ; Prieto, Evandro Luis ; Nicolela, Nicole Castro Silva ; da Silva Marin, Tamiris Garcia ; dos Santos, Talita Alves ; de Oliveira da Silva, Joao Pedro Maia ; da Cunha, Anderson Ferreira ; Behlau, Franklin ; Novo-Mansur, Maria Teresa Marques

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 14, p. 17-pg., 2023-07-01.

Resumo

Citrus canker, caused by the bacterium Xanthomonas citri (Xcc), is one of the most devastating diseases for the citrus industry. Xylose is a constituent of the cell wall of plants, and the ability of Xcc to use this carbohydrate may play a role in virulence. Xcc has two genes codifying for xylose isomerase (XI), a bifunctional enzyme that interconverts D-xylose into D-xylulose and D-glucose into D-fructose. The aim of this work was to investigate the functional role of the two putative XI ORFs, XAC1776 (xylA1) and XAC4225 (xylA2), in Xcc pathogenicity. XI-coding genes of Xcc were deleted, and the single mutants (Xcc & UDelta;xylA1 or Xcc & UDelta;xylA2) or the double mutant (Xcc & UDelta;xylA1 & UDelta;xylA2) remained viable. The deletion of one or both XI genes (xylA1 and/or xylA2) increased the aggressiveness of the mutants, causing disease symptoms. RT-qPCR analysis of wild strain and xylA deletion mutants grown in vivo and in vitro revealed that the highest expression level of hrpX and xylR was observed in vivo for the double mutant. The results indicate that XI depletion increases the expression of the hrp regulatory genes in Xcc. We concluded that the intracellular accumulation of xylose enhances Xcc virulence. (AU)

• Processo FAPESP: 21/14224-4 - Análise da expressão de xilose isomerase (XylA) e de genes hrp em Xanthomonas citri subsp. citri in vivo por qRT-PCR e análise proteômica
• Beneficiário: Evandro Luis Prieto
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 20/05529-3 - Análise funcional dos genes xanB e xylA potencialmente envolvidos com a patogenicidade de Xanthomonas citri subsp. citri
• Beneficiário: Maria Teresa Marques Novo Mansur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/50910-2 - Análise proteômica diferencial em Xanthomonas axonopodis: proteínas e genes de interesse biotecnológico
• Beneficiário: Maria Teresa Marques Novo Mansur
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/17470-0 - Análise funcional da xilose isomerase na resposta à xilose por Xanthomonas citri e X. fuscans- tipo B e análise proteômica diferencial desta resposta
• Beneficiário: Evandro Luis Prieto
• Modalidade de apoio: Bolsas no Brasil - Doutorado