| Texto completo | |
| Autor(es): |
Donadon, Larissa Gabriella Fusco
;
Salata, Giovanna Cassone
;
Goncalves, Thalita Pedralino
;
Matos, Lisa de Carvalho
;
Evangelista, Maria Clara Paiva
;
da Silva, Nicole Sampaio
;
Martins, Tereza Silva
;
Machado-Neto, Joao Agostinho
;
Lopes, Luciana Biagini
;
Garcia, Maria Teresa Junqueira
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Pharmaceutics; v. 633, p. 18-pg., 2023-02-01. |
| Resumo | |
This study evaluated the potential of monoolein (MO)-based nanodispersions to promote the cutaneous co -delivery of metformin (MET) and methylene blue (MB) for the treatment of non-melanoma skin cancer. MO -based nanodispersions were obtained using Kolliphor (R) P407 (KP) and/or sodium cholate (CH), and character-ized concerning the structure, thermal stability, ability to disrupt the skin barrier, cutaneous permeation and retention of MB and MET. Additionally, the cytotoxic effect of MO nanodispersions-mediated combination therapy using MET and MB in A431 cells was evaluated. The nanodispersions exhibited nanometric size (<200 nm) and thermal and physical stability. Small angle X-ray scattering studies revealed multiple structures depending on composition. They were able to interact with stratum corneum lipid structure, increasing its fluidity. The effect of MO-nanodispersions on topical/transdermal delivery of MB and MET was composition -dependent. Nanodispersions with low MO content (5 %) and stabilized with KP and CH (0.05-0.10 %) were the most promising, enhancing the cutaneous delivery of MB and MET by 1.9 to 2.2-fold and 1.4 to 1.7-fold, respectively, compared to control. Cytotoxic studies revealed that the most promising MO nanodispersion-mediated combination therapy using MET and MB (1:1) reduced the IC50 by 24-fold, compared to MB solu-tion, and a further reduction (1.5-fold) was observed by MB photoactivation. (AU) | |
| Processo FAPESP: | 17/18239-0 - Nanoemulsões como sistemas de liberação do azul de metileno e da metformina para tratamento do câncer de pele: estudos de liberação, permeação/retenção 'in vitro' e citotoxicidade. |
| Beneficiário: | Maria Teresa Junqueira Garcia |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/13877-1 - Nanocarreadores para a quimioprevenção e tratamento localizado de tumores de mama |
| Beneficiário: | Luciana Biagini Lopes |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 17/17844-8 - Sílica nanoestruturada como veículo protetor de vacinas e biomoléculas |
| Beneficiário: | Osvaldo Augusto Brazil Esteves Sant'Anna |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/08582-5 - EMU concedido no processo 17/17844-8:Analisador térmico - TG/DTA modelo sta 2500 TG/DTA TEMP. ambiente a 1100 °c |
| Beneficiário: | Tereza da Silva Martins |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 19/26048-6 - Desenvolvimento e aplicação de nanocarreadores multifuncionais para entrega intraductal de quimioterápicos visando o tratamento do Câncer de Mama |
| Beneficiário: | Giovanna Cassone Salata |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |