| Texto completo | |
| Autor(es): |
Teixeira-Fonseca, Jorge Lucas
;
Joviano-Santos, Julliane V.
;
Beserra, Samuel Santos
;
Conceicao, Michael Ramon de Lima
;
Leal-Silva, Polyana
;
Marques, Leisiane Pereira
;
Souza, Diego Santos
;
Roman-Campos, Danilo
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY; v. N/A, p. 14-pg., 2023-06-20. |
| Resumo | |
The TASK-1 channel belongs to the two-pore domain potassium channel family. It is expressed in several cells of the heart, including the right atrial (RA) cardiomyocytes and the sinus node, and TASK-1 channel has been implicated in the pathogenesis of atrial arrhythmias (AA). Thus, using the rat model of monocrotaline-induced pulmonary hypertension (MCT-PH), we explored the involvement of TASK-1 in AA. Four-week-old male Wistar rats were injected with 50 mg/kg of MCT to induce MCT-PH and isolated RA function was studied 14 days later. Additionally, isolated RA from six-week-old male Wistar rats were used to explore the ability of ML365, a selective blocker of TASK-1, to modulate RA function. The hearts developed right atrial and ventricular hypertrophy, inflammatory infiltrate and the surface ECG demonstrated increased P wave duration and QT interval, which are markers of MCT-PH. The isolated RA from the MCT animals showed enhanced chronotropism, faster contraction and relaxation kinetics, and a higher sensibility to extracellular acidification. However, the addition of ML365 to extracellular media was not able to restore the phenotype. Using a burst pacing protocol, the RA from MCT animals were more susceptible to develop AA, and simultaneous administration of carbachol and ML365 enhanced AA, suggesting the involvement of TASK-1 in AA induced by MCT. TASK-1 does not play a key role in the chronotropism and inotropism of healthy and diseased RA; however, it may play a role in AA in the MCT-PH model. (AU) | |
| Processo FAPESP: | 19/21304-4 - Mecanismos arritmogênicos em doenças do coração direito |
| Beneficiário: | Danilo Roman Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/18918-0 - Participação do óxido nítrico nas arritmias cardíacas durante o Hipotireoidismo |
| Beneficiário: | Diego Santos de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/15122-0 - Cardiotoxicidade induzida pelo pesticida tebuconazol em camundongos: da concepção a vida adulta |
| Beneficiário: | Leisiane Pereira Marques |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 20/14635-1 - Modelagem de doenças monogênicas para estudos fisiopatológicos e testes farmacológicos utilizando células especializadas derivadas de iPSCs |
| Beneficiário: | João Bosco Pesquero |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/05584-7 - Avaliação da toxicidade aguda e crônica ao coração e ao cérebro expostos a pesticidas encontrados na água potável do Brasil |
| Beneficiário: | Danilo Roman Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |