| Texto completo | |
| Autor(es): |
Rocha, Bruno Alves
;
Gallimberti, Matheus
;
Ximenez, Joao Paulo Bianchi
;
Basso, Carla Giovana
;
Martino-Andrade, Anderson Joel
;
Koch, Holger Martin
;
Calixto, Leandro Augusto
;
Barbosa, Fernando
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 266, p. 10-pg., 2024-01-01. |
| Resumo | |
Urinary phthalate metabolite (mPAEs) analysis is a reliable tool for assessing human exposure to phthalates. With growing interest in urinary biomonitoring of these metabolites, there is a need for fast and sensitive analytical methods. Therefore, a simple, rapid procedure for simultaneous determination of fifteen phthalate metabolites in human urine samples by liquid chromatography-tandem mass spectrometry was developed. The novelty of the present procedure is based on the use of diethyl carbonate as a green biobased extraction solvent and air-assisted liquid-liquid microextraction (AALLME) as a sample preparation step. A Plackett-Burman design was used for screening the factors that influence the AALLME extraction efficiency of mPAEs. The effective factors were then optimized by response surface methodology using a central composite rotatable design. Under the optimized conditions, good linearity can be achieved in a concentration range of 1.0-20.0 ng mL-1 with correlation coefficients higher than 0.99. The repeatability and reproducibility precision were in the range of 2-12% and 1-10% respectively. Recoveries ranging from 90% to 110%. This, and the low limits of detection, ranging from 0.01 to 0.05 ng mL-1, make the proposed procedure sensitive and suitable for human biomonitoring of phthalate exposures. For proof-of-principle, the new method was used to measure the urinary concentrations of mPAEs in 20 urine samples from Brazilian women. The high frequency of detections and in part high con-centrations of mPAEs indicate to widespread exposure to several phthalates among Brazilian women. (AU) | |
| Processo FAPESP: | 22/07979-1 - Metabolômica do expossoma por espectrometria de massas |
| Beneficiário: | Bruno Alves Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 21/03633-0 - Desenvolvimento e aplicação de métodos analíticos simples e rápidos para a determinação de desreguladores endócrinos e biomarcadores de estresse oxidativo em amostras biológicas para avaliação da exposição de crianças e gestantes |
| Beneficiário: | Bruno Alves Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua |
| Beneficiário: | Fernando Barbosa Júnior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/02939-6 - Análise sistemática da relação dinâmica entre ambiente, metabolismo e condição de saúde, aplicando uma abordagem de aprendizado de máquina |
| Beneficiário: | João Paulo Bianchi Ximenez |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |