| Texto completo | |
| Autor(es): Mostrar menos - |
Peluso, A. Augusto
;
Lundgaard, Agnete T.
;
Babaei, Parizad
;
Mousovich-Neto, Felippe
;
Rocha, Andrea L.
;
Damgaard, Mads V.
;
Bak, Emilie G.
;
Gnanasekaran, Thiyagarajan
;
Dollerup, Ole L.
;
Trammell, Samuel A. J.
;
Nielsen, Thomas S.
;
Kern, Timo
;
Abild, Caroline B.
;
Sulek, Karolina
;
Ma, Tao
;
Gerhart-Hines, Zach
;
Gillum, Matthew P.
;
Arumugam, Manimozhiyan
;
Orskov, Cathrine
;
McCloskey, Douglas
;
Jessen, Niels
;
Herrgard, Markus J.
;
Mori, Marcelo A. S.
;
Treebak, Jonas T.
Número total de Autores: 24
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NPJ AGING; v. 9, n. 1, p. 21-pg., 2023-04-03. |
| Resumo | |
The gut microbiota impacts systemic levels of multiple metabolites including NAD(+) precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD(+) precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context. (AU) | |
| Processo FAPESP: | 16/12294-7 - Papel de Dicer em adipócitos controlando a função imune e a ativação das células beges no tecido adiposo |
| Beneficiário: | Andréa Livia Silva Rocha |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/01184-9 - CAMeLEOm: análise entre espécies dos efeitos metabólicos, na expectativa de vida e ômicas de miméticos de restrição dietética |
| Beneficiário: | Marcelo Alves da Silva Mori |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/11672-3 - Caracterização em camundongos de novos genes envolvidos nos mecanismos evolutivamente conservados da restrição dietética e seus miméticos |
| Beneficiário: | Felippe Mousovich Neto |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |