Selection and Control of Process Conditions Enable the Preparation of Curcumin-Loaded Poly(lactic-co-glycolic acid) Nanoparticles of Superior Performance

Autor(es)

da Silva Feltrin, Felipe ; D'Angelo, Natalia Aimee ; de Oliveira Guarnieri, Joao Paulo ; Lopes, Andre Moreni ; Lancellotti, Marcelo ; Lona, Liliane Maria Ferrareso

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 22, p. 14-pg., 2023-05-23.

Resumo

Curcumin (CUR) is one natural bioactive compound acknowledgedfordiverse therapeutic activities, but its use is hindered by its poorbioavailability, fast metabolism, and susceptibility to pH variationsand light exposure. Thus, the encapsulation in poly-(lactic-co-glycolic acid), or PLGA, has been successfully used toprotect and enhance CUR absorption in the organism, making CUR-loadedPLGA nanoparticles (NPs) promising drug delivery systems. However,few studies have focused beyond CUR bioavailability, on the environmentalvariables involved in the encapsulation process, and whether theycould help obtain NPs of superior performance. Our study evaluatedpH (3.0 or 7.0), temperature (15 or 35 degrees C), light exposure, andinert atmosphere (N-2) incidence in the encapsulation ofCUR. The best outcome was at pH 3.0, 15 degrees C, without light incidence,and without N-2 usage. This best nanoformulation showedNP size, zeta potential, and encapsulation efficiency (EE) of 297nm, -21 mV, and 72%, respectively. Moreover, the CUR in vitro release at pH values 5.5 and 7.4 suggested differentpotential applications for these NPs, one of which was demonstratedby the effective inhibition of multiple bacteria (i.e., Gram-negative, Gram-positive, and multi-resistant) in the minimalinhibition concentration assay. Besides, statistical analyses confirmeda significant impact of temperature on the NP size; in addition, temperature,light, and N-2 affected the EE of CUR. Thus, the selectionand control of process variables resulted in higher CUR encapsulationand customizable outcomes, ultimately enabling more economical processesand providing future scale-up guidelines. (AU)

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