Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil

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Cunha, Lia ; Luchs, Adriana ; Azevedo, Lais S. S. ; Silva, Vanessa C. M. ; Lemos, Marcilio F. F. ; Costa, Antonio C. C. ; Compri, Adriana P. P. ; Franca, Yasmin ; Viana, Ellen ; Malta, Fernanda ; Medeiros, Roberta S. S. ; Guiducci, Raquel ; Morillo, Simone G. G. ; Gomes-Gouvea, Michele S. S. ; Amgarten, Deyvid ; Pinho, Joao R. R. ; Moreira, Regina C. C.

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 15, n. 2, p. 13-pg., 2023-02-01.

Resumo

Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in Sao Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7-93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide. (AU)

• Processo FAPESP: 20/02469-0 - Constelação completa dos genótipos de cinco cepas raras de rotavírus g12p[9] detectadas no Brasil em 2011-2013
• Beneficiário: Yasmin França Viana Pires de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/12944-9 - Investigando a evolução de cepas animais de rotavírus infectando humanos
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/14786-0 - Vigilância e epidemiologia molecular dos Bocavirus humanos associados à gastroenterite aguda
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/11182-6 - Epidemiologia molecular de cepas de Rotavirus G8 isoladas no Brasil associadas aos genótipos p[4], p[6] ou p[8]
• Beneficiário: Roberta Salzone Medeiros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica