Applying CeO2 nanorods in flexible electrochemical immunosensor to detect C-reactive protein

Autor(es)

Amoresi, Rafael Aparecido Ciola ; Roza, Noemi Angelica Vieira ; Mazon, Talita

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ELECTROANALYTICAL CHEMISTRY; v. 935, p. 9-pg., 2023-03-22.

Resumo

In this work, we report the development of a flexible electrochemical immunosensor based on CeO2 nanorods (NRs) immobilized with C-reactive protein (CRP) antibody, a biomarker of cardiovascular disease. CeO2 NRs were obtained by microwave-assisted hydrothermal method and characterized by XRD, TEM, and XPS. For the preparation of the working electrode, WE, of the immunosensor, the NRs were dispersed in organic solvent and then functionalized with cystamine and glutaraldehyde, and then modified with CRP antibody. The depo- sition condition was evaluated for electrode functionalization and immobilization. Functionalization and immobilization were verified by spectroscopic techniques and proved effective repeatability and reproducibil- ity. The developed immunosensor had acceptable reproducibility (coefficient of variation=4.5 %), allowed detection in the range of 0.3 to 7.0 mg L-1 of CRP with a detection limit of 0.18 mg L-1, and applications that are immediately detectable, easy-to-handle, low-cost and ideal for point-of-care. (AU)

• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/19143-7 - Uma abordagem teórico-experimental dos mecanismos de condução e defeitos de interface em heteroestruturas sensoras baseados em semicondutores P-N e N-N a partir de matrizes de CeO2
• Beneficiário: Rafael Aparecido Ciola Amoresi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado