Biodegradable and Flexible Thermoplastic Composite Graphite Electrodes: A Promising Platform for Inexpensive and Sensitive Electrochemical Detection of Creatine Kinase at the Point-of-Care

Autor(es)

de Lima, Lucas Felipe ; Ferreira, Andre Lopes ; de Freitas, Amanda de Sousa Martinez ; Rodrigues, Jessica de Souza ; Lemes, Ana Paula ; Ferreira, Marystela ; de Araujo, William Reis

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 15, p. 13-pg., 2023-04-04.

Resumo

Acute myocardial infarction (AMI) is the main cause of death worldwide, and the time of diagnosis is decisive for the effectiveness of the treatment of patients with AMI. Creatine kinase-myocardial band (CK-MB) has a predominance and high affinity with myocardial tissue, making it considered one of the main biomarkers for the diagnosis of AMI. In this work, we report a novel biodegradable composite material based on a polymer blend of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Poly(butylene adipate-co-terephthalate) (PHBV:Ecoflex) and graphite microparticles for sensitive and selective electrochemical detection of CK-MB. The morphological and physicochemical characterizations of the thermoplastic composite material revealed a homogeneous and synergistic distribution of the graphite microparticles through the blend structure, providing low defects and high electrical conductivity with high electron transfer kinetics (k0 = 3.54 x 10-3 cm s-1) features with adequate flexibility for point-of-care applications. The portable and disposable devices were applied to detect CK-MB using the electrochemical impedance spectroscopy (EIS) technique in a relevant clinical concentration ranging from 5.0 ng mL-1 to 100.0 ng mL-1 and presented a limit of detection of 0.26 ng mL-1 CK-MB. The selectivity of the sensor was confirmed by testing the potential interference of major biomolecules found in biofluids and other relevant macromolecules. The accuracy and robustness were assessed by addition and recovery protocol in urine and saliva samples without sample pretreatment and demonstrated the potential of our method for rapid and decentralized tests of AMI. In addition, the study of the thermal, biological, and photodegradation of the devices after being used was also carried out, aiming at the disposal of the material more sustainably. (AU)

• Processo FAPESP: 20/12659-0 - Utilização de poli (adipato-co-tereftalato de butileno) (PBAT) e materiais de carbono na fabricação de dispositivos eletroquímicos flexíveis e biodegradáveis
• Beneficiário: Marystela Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/19387-0 - Desenvolvimento de filmes nanoestruturados automontados (layer-by-layer) e langmuir-blodgett (lb) incorporando emodina para sistemas carreadores
• Beneficiário: Marystela Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/02692-3 - Desenvolvimento de um biossensor óptico pela técnica de ressonância plasmônica de superfície (SPR) para a determinação de creatina kinase (CK-MB)
• Beneficiário: André Lopes Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais
• Beneficiário: William Reis de Araujo
• Modalidade de apoio: Auxílio à Pesquisa - Projeto Inicial
• Processo FAPESP: 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais
• Beneficiário: Mauro Bertotti
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/04061-8 - Filmes automontados de polipirrol e nanopartículas de magnetita na determinação analítica de parabenos
• Beneficiário: Lucas Felipe de Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado