Leptospira sanjuanensis sp. nov., a pathogenic species of the genus Leptospira isolated from soil in Puerto Rico

Autor(es)

Fernandes, Luis G., V ; Stone, Nathan E. ; Roe, Chandler C. ; Goris, Marga G. A. ; van der Linden, Hans ; Sahl, Jason W. ; Wagner, David M. ; Nally, Jarlath E.

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 72, n. 10, p. 9-pg., 2022-01-01.

Resumo

Two spirochetes (designated strains LGVF01 and LGVF02T) were isolated from soil samples in San Juan, Puerto Rico in HANmedia after selection using a combination of ELISA, agar plating, and colony screening by Fluorescent Antibody Testing (FAT) and PCR for lipL32 and secY. Isolates were helix--shaped, aerobic, fast-growing, and highly motile. Genome sequence analysis indicated that both strains should be classified as members of a novel species within the pathogenic (P1) clade of the genus Leptospira. The average nucleotide identity between the two strains was 99.2%, but below 93.2 % when compared to any previously described leptospiral species. Serotyping of strain LGVF02T indicates that it does not belong within any serogroup of Leptospira suggesting it also represents a new serovar. Collectively, strains LGVF01 and LGVF02T represent a new species of pathogenic leptospires for which the name Leptospira sanjuanensis sp. nov. is proposed. The type strain is LGVF02T (=NVSL-LGVF02(T)=KIT0302T). (AU)

• Processo FAPESP: 19/20302-8 - Silenciamento de RNA não-codificador (ncRNA) mediado por interferência CRISPR (CRISPRi) em cepas saprofíticas e patogênicas de Leptospira
• Beneficiário: Luis Guilherme Virgílio Fernandes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/06731-8 - Aplicação de CRISPR-interferência na elucidação da patogênese da leptospirose e desenvolvimento de novas estratégias para obtenção de mutantes knockout
• Beneficiário: Luis Guilherme Virgílio Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado