| Texto completo | |
| Autor(es): |
Barbeiro, Camila de Oliveira
;
Fernandes, Darcy
;
Palacon, Mariana Paravani
;
Castilho, Rogerio Moraes
;
de Almeida, Luciana Yamamoto
;
Bufalino, Andreia
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF PERSONALIZED MEDICINE; v. 13, n. 4, p. 15-pg., 2023-04-01. |
| Resumo | |
Oral potentially malignant disorders (OPMD) are clinical presentations that carry an increased risk of cancer development. Currently, epithelial dysplasia grade is based on architectural and cytological epithelial changes and is used to predict the malignant transformation of these lesions. However, predicting which OPMD will progress to a malignant tumor is very challenging. Inflammatory infiltrates can favor cancer development, and recent studies suggest that this association with OPMD lesions may be related to the etiology and/or aggressive clinical behavior of these lesions. Epigenetic changes such as histone modifications may mediate chronic inflammation and also favor tumor cells in immune resistance and evasion. This study aimed to evaluate the relationship between histone acetylation (H3K9ac) and DNA damage in the context of dysplastic lesions with prominent chronic inflammation. Immunofluorescence of "low-risk" and "high-risk" OPMD lesions (n = 24) and inflammatory fibrous hyperplasia (n = 10) as the control group was performed to assess histone acetylation levels and DNA damage through the phosphorylation of H2AX (gamma H2AX). Cell co-culture assays with PBMCs and oral keratinocyte cell lines (NOK-SI, DOK, and SCC-25) were performed to assess proliferation, adhesion, migration, and epithelial-mesenchymal transition (EMT). Oral dysplastic lesions showed a hypoacetylation of H3K9 and low levels of gamma H2AX compared to control. The contact of dysplastic oral keratinocytes with PBMCs favored EMT and the loss of cell-cell adhesion. On the other hand, p27 levels increased and cyclin E decreased in DOK, indicating cell cycle arrest. We conclude that the presence of chronic inflammation associated to dysplastic lesions is capable of promoting epigenetic alterations, which in turn can favor the process of malignant transformation. (AU) | |
| Processo FAPESP: | 18/04954-2 - Análise da acetilação de Histona H3 e sua contribuição para a instabilidade genômica na leucoplasia oral e Leucoplasia Verrucosa Proliferativa |
| Beneficiário: | Camila de Oliveira Barbeiro |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 22/14672-0 - Estudo do mecanismo de evasão imune na Leucoplasia Verrucosa proliferativa |
| Beneficiário: | Andreia Bufalino |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/22236-0 - Análise comparativa do infiltrado linfocítico em leucoplasia verrucosa proliferativa e líquen plano oral. |
| Beneficiário: | Mariana Paravani Palaçon |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/01798-7 - Estudo in vitro da modulação da imunidade inata e adaptativa por queratinócitos displásicos |
| Beneficiário: | Darcy Fernandes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/01438-0 - Análise do papel da resposta imune inata e adaptativa na leucoplasia oral e leucoplasia verrucosa proliferativa |
| Beneficiário: | Andreia Bufalino |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |