Ontogenetic Variation in Ammonia Excretion during the Early Life Stages of the Amazon River Prawn, Macrobrachium amazonicum

Autor(es)

de Arruda Hayd, Liliam ; Lemos, Daniel ; Valenti, Wagner C.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE WORLD AQUACULTURE SOCIETY; v. 41, p. 9-pg., 2010-02-01.

Resumo

Dry mass (DM) and total ammonia-N (TAN) excretion were determined in embryos, larvae (ZI-ZIX, Z = zoea ), and postlarvae (PL) at 1, 7, and 14 d after metamorphosis (PL1, PL7, and PL14) of Macrobrachium amazonicum. Animals in postmolt-intermolt (A-C) stages were sorted according to their developmental stages, and placed into incubation chambers (similar to 30 mL) for 2 h to quantify TAN excretion. After this period, analyses were carried out using Koroleff's method for TAN determination. Individual TAN excretion generally increased throughout ontogenetic development and varied from 0.0090 +/- 0.0039 mu g TAN/individual/h in embryo to 1.041 +/- 0.249 mu g TAN/individual/h in PL14. There was no significant difference between embryo-ZIV and ZV-ZIX (P > 0.05), whereas PL1, PL7, and PL14 differed (P < 0.05) from each other. Higher increments in individual ammonia-N excretion were observed between ZIV-ZV, PL1-PL7, and PL7-PL14. Mass-specific excretion rates presented two groups, embryo-ZII (P > 0.05) and ZIII-PL14 (P > 0.05). The lowest value was found in embryo (0.17 +/- 0.07 mu g TAN/mg DM/h) and the maximum values in ZV and PL1 (0.65 +/- 0.25 and 0.64 +/- 0.27 mu g TAN/mg DM/h, respectively). Results indicate that metabolic rate is proportional to the body mass in M. amazonicum, during early life stages. Variations in ammonia excretion during this phase may be associated mainly with body size. Data obtained in the present study may be useful in developing and optimizing rearing techniques of M. amazonicum, such as the proportions between biofilter and rearing tank size, and stocking density in culture tanks or in transport bags. (AU)

• Processo FAPESP: 07/07051-9 - Digestibilidade in vitro de proteína utilizando enzimas espécie específicas: potencial de identificação de ingredientes de reação sustentáveis para o cultivo de camarões marinhos (Sustain-Ing)
• Beneficiário: Daniel Eduardo Lavanholi de Lemos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/50578-2 - Testando a qualidade de racoes para o cultivo de camaroes marinhos: validacao do metodo in vitro ph-stat por meio de ensaios in vivo de digestibilidade proteica (ezfeed).
• Beneficiário: Daniel Eduardo Lavanholi de Lemos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/54276-0 - Ciclo de muda, metabolismo respiratorio e avaliacao dos compostos nitrogenados em larvas do camarao-da-amazonia, macrobrachium amazonicum.
• Beneficiário: Wagner Cotroni Valenti
• Modalidade de apoio: Auxílio à Pesquisa - Regular