Central NO-cGMP pathway in thermoregulation and survival rate during polymicrobial sepsis

Autor(es)

Oliveira-Pelegrin, G. R. ; Branco, L. G. S. ; Rocha, M. J. A.

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Physiology and Pharmacology; v. 88, n. 2, p. 8-pg., 2010-02-01.

Resumo

Sepsis induces production of inflammatory mediators such as nitric oxide (NO) and causes physiological alterations, including changes in body temperature (T-b). We evaluated the involvement of the central NO cGMP pathway in thermoregulation during sepsis induced by cecal ligation and puncture (CLP), and analyzed its effect on survival rate. Male Wistar rats with a T-b probe inserted in their abdomen were intracerebroventricularly injected with 1 mu L N-G-nitro-L-arginine methyl ester (L-NAME, 250 mu g), a nonselective NO synthase (NOS) inhibitor; or aminoguanidine (250 mu g), an inducible NOS inhibitor; or 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ, 0.25 mu g), a guanylate cyclase inhibitor. Thirty minutes after injection, sepsis was induced by cecal ligation and puncture (CLP), or the rats were sham operated. The animals were divided into 2 groups for determination of T-b for 24 h and assessment of survival during 3 days. The drop in T-b seen in the CLP group was attenuated by pretreatment with the NOS inhibitors (p < 0.05) and blocked with ODQ. CLP rats pretreated with either of the inhibitors showed higher survival rates than vehicle injected groups (p < 0.05), and were even higher in the ODQ pretreated group. Our results showed that the effect of NOS inhibition on the hypothermic response to CLP is consistent with the role of nitrergic pathways in thermoregulation. (AU)

• Processo FAPESP: 05/50162-0 - Avaliação da participação do óxido nítrico na síntese de vasopressina e ocitocina durante choque séptico experimental induzido por ligadura e perfuração cecal
• Beneficiário: Gabriela Ravanelli de Oliveira Pelegrin
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/08532-0 - Alterações neuroendócrinas durante sepse experimental induzida por ligação cecal e punção
• Beneficiário: Maria José Alves da Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Regular