| Texto completo | |
| Autor(es): |
Leal, Marcelo Larciprete
;
Lamas, Leonardo
;
Aoki, Marcelo Saldanha
;
Ugrinowitsch, Carlos
;
Carneiro Ramos, Marcela Sorelli
;
Tricoli, Valmor
;
Moriscot, Anselmo Sigari
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY; v. 111, n. 10, p. 11-pg., 2011-10-01. |
| Resumo | |
The purpose of the present study was to evaluate the effects of 8 weeks of strength and power training on the expression of genes related to the canonical WNT pathway and beta-catenin protein levels in physically active men. Twenty-five subjects (27.4 +/- A 4.6 years) were balanced based on their relative maximum strength in the squat exercise (squat 1RM/body mass) and randomly assigned to strength training (ST) (n = 10), power training (PT) (n = 10), and control (C) (n = 5) groups. The ST and the PT groups performed high and low intensity squats, respectively, thrice a week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. Relative strength and power increased similarly in both ST and PT groups (P < 0.001). Fiber cross-sectional area also increased similarly in both ST and PT groups. Gene expression and beta-catenin protein expression levels were assessed by real-time PCR and Western blot. Certain genes were up-regulated in the ST group (WNT1: 6.4-fold, P < 0.0001; SFRP1: 3.3-fold, P < 0.0001 and LEF1: 7.3-fold, P < 0.0001) and also in the PT group (WNT1: 24.9-fold, P < 0.0001; SFRP1: 2.7-fold, P < 0.0001; LEF1: 34.1-fold, P < 0.0001 and Cyclin D1: 7.7-fold, P < 0.001). In addition, the expression of key WNT pathway genes was substantially more responsive to PT than to ST (WNT1: P < 0.0001; LEF1: P < 0.0001 and Cyclin D1: P < 0.001). Finally, the total beta-catenin protein content increased only in the PT group (P < 0.05). Our data indicate that a PT regimen triggers greater responses in key elements of the WNT pathway. (AU) | |
| Processo FAPESP: | 06/00302-3 - Treinamento força com vibração: análise dos fatores determinantes da melhora no desempenho do salto vertical |
| Beneficiário: | Carlos Ugrinowitsch |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/52288-7 - Efeito de diferentes protocolos de treinamento de força sobre a expressão de genes da via WNT em humanos |
| Beneficiário: | Marcelo Larciprete Leal |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 06/61523-7 - Aspectos celulares e moleculares da plasticidade muscular |
| Beneficiário: | Anselmo Sigari Moriscot |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 08/58415-3 - Expressao de genes envolvidos na sinalizacao da miostatina (gdf-8) em resposta a diferentes modelos de treinamento de forca. |
| Beneficiário: | Marcelo Saldanha Aoki |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |