Epicatechin Used in the Treatment of Intestinal Inflammatory Disease: An Analysis by Experimental Models

Autor(es)

de Paula Vasconcelos, Paulo Cesar ; Seito, Leonardo Noboru ; Di Stasi, Luiz Claudio ; Hiruma-Lima, Clelia Akiko ; Pellizzon, Claudia Helena

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2012, p. 12-pg., 2012-01-01.

Resumo

Background. This study was pathway of (-)-epicatechin (EC) in the prevention and treatment of intestine inflammation in acute and chronic rat models. Methods. Intestine inflammation was induced in rats using TNBS. The morphological, inflammatory, immunohistochemical, and immunoblotting characteristics of colon samples were examined. The effects of EC were evaluated in an acute model at doses of 5, 10, 25, and 50 mg/kg by gavage for 5 days. The chronic colitis model was induced 1st day, and treated for 21 days. For the colitis relapse model, the induction was repeated on 14th. Results. EC10 and EC50 effectively reduced the lesion size, as assessed macroscopically; and confirmed by microscopy for EC10. The glutathione levels were higher in EC10 group but decreased COX-2 expression and increased cell proliferation (PC) were observed, indicating an anti-inflammatory activity and a proliferation-stimulating effect. In the chronic colitis model, EC10 showed lower macroscopic and microscopic lesion scores and increase in glutathione levels. As in the acute model, a decrease in COX-2 expression and an increase in PC in EC10, the chronic model this increase maybe by the pathway EGF expression. Conclusion. These results confirm the activity of EC as an antioxidant that reduces of the lesion and that has the potential to stimulate tissue healing, indicating useful for preventing and treating intestine inflammation. (AU)

• Processo FAPESP: 06/55542-9 - Avaliação do potencial da flora do cerrado em tratamento de lesões de mucosa gástrica e intestinal em modelos experimentais
• Beneficiário: Claudia Helena Pellizzon
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/05503-6 - Uso sustentável da biodiversidade brasileira: prospecção químico-farmacológica em plantas superiores
• Beneficiário: Wagner Vilegas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 07/53201-2 - Efeito da (-)-epicatequina presente nas folhas Mouriri pusa Gardn. (Melastomataceae) na prevenção e tratamento de colite ulcerativa em ratos
• Beneficiário: Paulo César de Paula Vasconcelos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/08536-9 - Atividade gastroprotetora do mentol e da epicatequina: verificação dos mecanismos de ação sob visões morfológica, fisiológica e farmacológica
• Beneficiário: Claudia Helena Pellizzon
• Modalidade de apoio: Auxílio à Pesquisa - Regular