Genotypic Characterization of Yersinia enterocolitica Biotype 4/O:3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE

Autor(es)

Paixao, Renata ; Moreno, Luisa Zanolli ; Sena de Gobbi, Debora Dirani ; Raimundo, Daniele Cristine ; Porfida Ferreira, Thais Sebastiana ; Spindola, Maria Garcia ; Hofer, Ernesto ; Falavina dos Reis, Cristhiane Moura ; Matte, Maria Helena ; Moreno, Andrea Micke

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PATHOGENS; v. 2013, p. 8-pg., 2013-01-01.

Resumo

Yersinia enterocolitica is a foodborne pathogen that causes illness in humans and animals. The biotype 4/O:3 has been commonly associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes. Molecular typing methods have been successfully used to characterize Y. enterocolitica genetic heterogeneity and to study the epidemiology of the bacteria from different origins. In this study, 320 Y. enterocolitica biotype 4/O:3 isolates originating in pigs and slaughterhouses were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE techniques. The majority of the isolates originated from pigs, and the predominant virulence profile was ail+ virF+ rfbC+ ystA+, representing 83.4% of the tested isolates. All of the Y. enterocolitica 4/O:3 isolates were positive for at least ystA gene. The SE-AFLP and ERIC-PCR patterns were highly homogeneous. The SE-AFLP was more discriminative than the ERIC-PCR and tended to cluster isolates according to the slaughterhouse. Despite the limited genetic diversity of Y. enterocolitica 4/O:3, PFGE was shown to be the most discriminative technique considering one band of difference. Fattening pigs proved to be an important reservoir of Y. enterocolitica biotype 4/O:3 carrying virulence genes. (AU)

• Processo FAPESP: 06/55501-0 - Isolamento e caracterizacao de listeria monocytogenes e yersinia enterocolitica em abatedouros e cortes de carne suina no estado de sao paulo e sua comparacao genotipica com isolados de casos clinicos em..
• Beneficiário: Renata Paixão
• Modalidade de apoio: Bolsas no Brasil - Doutorado