Phenotypic and Genotypic Characterization of Atypical Listeria monocytogenes and Listeria innocua Isolated from Swine Slaughterhouses and Meat Markets

Autor(es)

Moreno, Luisa Zanolli ; Paixao, Renata ; Sena de Gobbi, Debora Dirani ; Raimundo, Daniele Cristine ; Porfida Ferreira, Thais Sebastiana ; Moreno, Andrea Micke ; Hofer, Ernesto ; Falavina dos Reis, Cristhiane Moura ; Matte, Glavur Rogerio ; Matte, Maria Helena

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2014, p. 12-pg., 2014-01-01.

Resumo

In the last decade, atypical Listeria monocytogenes and L. innocua strains have been detected in food and the environment. Because of mutations in the major virulence genes, these strains have different virulence intensities in eukaryotic cells. In this study, we performed phenotypic and genotypic characterization of atypical L. monocytogenes and L. innocua isolates obtained from swine slaughterhouses and meat markets. Forty strains were studied, including isolates of L. monocytogenes and L. innocua with low-hemolytic activity. The isolates were characterized using conventional phenotypic Listeria identification tests and by the detection and analysis of L. monocytogenes-specific genes. Analysis of 16S rRNA was used for the molecular identification of the Listeria species. The L. monocytogenes isolates were positive for all of the virulence genes studied. The atypical L. innocua strains were positive for hly, plcA, and inlC. Mutations in the InlC, InlB, InlA, PI-PLC, PC-PLC, and PrfA proteins were detected in the atypical isolates. Further in vitro and transcriptomic studies are being developed to confirm the role of these mutations in Listeria virulence. (AU)

• Processo FAPESP: 10/13511-5 - Pesquisa de genes de virulência em cepas de Listeria monocytogenes e Listeria innocua originárias de ambiente e alimento
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/55501-0 - Isolamento e caracterizacao de listeria monocytogenes e yersinia enterocolitica em abatedouros e cortes de carne suina no estado de sao paulo e sua comparacao genotipica com isolados de casos clinicos em..
• Beneficiário: Renata Paixão
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/19005-4 - Identificação de genes de virulência e caracterização do perfil de resistência a antimicrobianos em cepas de Listeria spp. originárias de ambiente, alimento e infecção humana
• Beneficiário: Maria Helena Matte
• Modalidade de apoio: Auxílio à Pesquisa - Regular