Inhibition of Macrophage Functions by the C-Terminus of Murine S100A9 Is Dependent on B-1 Cells

Autor(es)

Pagano, Rosana Lima ; Moraes, Natassja Foizer ; De Lorenzo, Beatriz Helena ; Sampaio, Sandra Coccuzzo ; Mariano, Mario ; Giorgi, Renata

• Tipo de documento: Artigo Científico
• Fonte: Mediators of Inflammation; v. 2014, p. 10-pg., 2014-01-01.

Resumo

The protein S100A9 plays a key role in the control of inflammatory response. The C-terminus of the murine S100A9 protein (mS100A9p) downregulates the spreading and phagocytic activity of adherent peritoneal cells. Murine peritoneal cells are constituted bymacrophages and B-1 cells, and the latter exert an inhibitory effect on macrophage functions by secreting interleukin-(IL-) 10. Here, we investigated the influence of B-1 cells on the inhibitory effect evoked by mS100A9p on macrophages. mS100A9p did not alter spreading and phagocytosis either by peritoneal macrophages obtained from mice deprived of B-1 cells or by bone marrow-derived macrophages (BMDM phi). Nevertheless, when BMDM phi were cocultivated by direct or indirect contact with B-1 cells treated with mS100A9p, the phagocytosis by BMDM phi was decreased, showing that the effect of mS100A9p on macrophages was modulated by B-1 cells and/or their secretory compounds. Furthermore, the inhibitory action of mS100A9p on phagocytosis by adherent peritoneal cells was abolished in cells obtained from IL-10 knockout mice. Taken together, the results show that mS100A9p has no direct inhibitory effect on macrophages; however, mS100A9p modulates B-1 cells, which in turn downregulates macrophages, at least in part, via IL-10. These data contribute to the characterization of S100A9 functions involving B-1 cells in the regulation of the inflammatory process. (AU)

• Processo FAPESP: 02/08277-7 - Efeito "in vitro" do peptideo sintetico homologo a porcao c-terminal da proteina ligante de calcio mrp-14 murina sobre a funcionalidade de macrofagos peritoneais de camundongos.
• Beneficiário: Rosana de Lima Pagano
• Modalidade de apoio: Bolsas no Brasil - Doutorado