Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes

Autor(es)

Jeronimo, Rodrigo ; Moraes, Maria Nathalia ; Monteiro de Assis, Leonardo Vinicius ; Ramos, Bruno Cesar ; Rocha, Thaina ; de Lauro Castrucci, Ana Maria

• Tipo de documento: Artigo Científico
• Fonte: Journal of Thermal Biology; v. 68, p. 11-pg., 2017-08-01.

Resumo

It is believed that the biological systems perceiving temperature and light daily cycles were subjected to the simultaneous selective pressures, which resulted in their co-evolutionary association. We investigated the influence of 1 h 33 degrees C heat shock on the expression of clock and heat shock protein genes, as well as the role of the thermo-TRP channel, TRPV1, in ZEM-2S cells of the teleost Danio rerio, in constant dark (DD) or light-dark cycles (LD). After heat shock, we observed an acute increase of hsp90 aal levels in both DD and LD conditions. Interestingly, the expression of hsp90 aal was two-fold lower in LD than in DD, what suggests an antagonistic effect of white light on heat shock action. Regarding clock genes, no effect was found in cells subjected to the heat shock in DD. When cells were kept in LD, the expression of pent, per2, cryla, and crylb increased in response to heat shock, indicating that heat shock only affects clock core of LD-synchronized ZEM-2S cells. We then evaluated whether TRPV1 played a role in heat-mediated hsp90 aal and per2 responses: hsp90 aal increase was unaffected whereas per2 increase was partially blocked by TRPV1 inhibitor, demonstrating the channel participation in clock gene regulation by heat shock. Taken together, our results open a novel investigative perspective regarding the relationship between temperature and clock genes, placing a new player in the regulation of this phenomenon: the TRPV1 channel. (AU)

• Processo FAPESP: 12/50214-4 - Mecanismos de ajuste do relógio por luz e temperatura: aspectos filogenéticos
• Beneficiário: Ana Maria de Lauro Castrucci
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/16412-9 - Mecanismos de termo-modulação de genes de relógio em tecidos periféricos de mamíferos: papel dos canais TRP
• Beneficiário: Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/24337-4 - Mecanismos de modulação de genes de relógio em melanócitos normais (melan-A) e transformados (melanoma B16-F10) por UVA e luz azul
• Beneficiário: Leonardo Vinícius Monteiro de Assis
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto