Activity of 3 '-hydroxychalcone against Cryptococcus gattii and toxicity, and efficacy in alternative animal models

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Palanco, Ana Cerrejn ; Singulani, Junya de lacorte ; Costa-Orlandi, Caroline Barcelos ; Gullo, Fernanda Patricia ; Strohmayer Lourencetti, Natalia Manuela ; Gomes, Paulo Cesar ; Ayusso, Gabriela Miranda ; Dutra, Luiz Antonio ; Bolzani, Vanderlan da Silva ; Regasini, Luis Octavio ; Soares Mendes-Giannini, Maria Jose ; Fusco-Almeida, Ana Marisa

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 12, n. 13, p. 12-pg., 2017-10-01.

Resumo

Aim: This work aimed to evaluate the activity of 3'-hydroxychalcone against Cryptococcus gattii in planktonic and biofilm forms and their toxicity using alternative animal models. Materials & methods: Minimum inhibitory concentration and minimum fungicide concentration were determined. Biofilm formation and the susceptibility tests were performed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[carbonyl(phenylamino)]2H-tetrazolium hydroxide assay. Toxicity and efficacy were checked in Danio rerio and Galleria mellonella models. Results: The compound 3'-hydroxychalcone showed fungicidal activity against C. gattii in both planktonic and biofilm forms. The toxicity in zebrafish embryos revealed a low lethal concentration. In G. mellonella, the compound did not show antifungal activity and larvae toxicity. Conclusion: Because of the activity of 3'-hydroxychalcone against C. gattii in vitro, molecular modifications should be made to improve efficacy and to reduce toxicity in vivo. (AU)

• Processo FAPESP: 14/10446-9 - Pesquisa de novas terapias e de biomarcadores na paracoccidioidomicose: estudo da atividade antifúngica de galatos de alquila em modelos alternativos e camundongos e identificação de microRNAs circulantes
• Beneficiário: Junya de Lacorte Singulani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/18330-0 - Preparação e avaliação biológica de híbridos curcumina-cinamaldeído como inibidores da divisão celular bacteriana
• Beneficiário: Luis Octávio Regasini
• Modalidade de apoio: Auxílio à Pesquisa - Regular