Effect of Curcumin-Nanoemulsion Associated with Photodynamic Therapy in Cervical Carcinoma Cell Lines

Autor(es)

Adum de Matos, Renata Prandini ; Calmon, Marilia Freitas ; Amantino, Camila Fernanda ; Villa, Luisa Lina ; Primo, Fernando Lucas ; Tedesco, Antonio Claudio ; Rahal, Paula

• Tipo de documento: Artigo Científico
• Fonte: BIOMED RESEARCH INTERNATIONAL; v. 2018, p. 11-pg., 2018-01-01.

Resumo

Cervical cancer is the fourth cause of cancer death in women. Curcumin has antineoplastic properties. Furthermore, curcumin may be used as a photosensitizing agent in Photodynamic Therapy. This study aimed to investigate the effects of Photodynamic Therapy in cellular viability using curcumin-nanoemulsion as a photosensitizing drug in cervical carcinoma cell lines. Theempty nanoemulsion presented very lowcytotoxicity in all cell lines analyzed. Additionally, the incubation with curcumin-nanoemulsion at 20.. Mof curcumin showed more than 80% of cell viability for cell lines. Nanoemulsions were shown to be internalized inside cells by fluorescence microscopy and were observed in the intracellular environment for up to 36 hours after incubation with cell lines. In addition, after the Photodynamic Therapy we observed a high phototoxic effect of the curcumin-nanoemulsion with less than 5% of viable cells after irradiation. This was accompanied by an increase in caspase-3/caspase-7 activities after cell treatment with curcumin-nanoemulsion and Photodynamic Therapy, suggesting cell death by apoptosis. We conclude that the curcumin-nanoemulsion formulation behaves as a photosensitizing drug in Photodynamic Therapy and shows potential as an alternative treatment to cervical lesions using an endoscopic diode fiber laser setup for in situ activation or cavity activation using a diffuse fiber delivery system. (AU)

• Processo FAPESP: 13/04286-6 - Estudo da ação de Nanopartículas associadas à curcumina em células de carcinoma cervical positivo para HPV
• Beneficiário: Renata Prandini Adum de Matos
• Modalidade de apoio: Bolsas no Brasil - Doutorado