| Texto completo | |
| Autor(es): Mostrar menos - |
Ribeiro, Liliane F. C.
;
Chelius, Cynthia
;
Boppidi, Karthik R.
;
Naik, Nisha S.
;
Hossain, Simin
;
Ramsey, Jessica J. J.
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Kumar, Jyothi
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Ribeiro, Lucas F.
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Ostermeier, Marc
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Bao Tran
;
Goo, Young Ah
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de Assis, Leandro J.
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Ulas, Mevlut
;
Bayram, Ozgur
;
Goldman, Gustavo H.
;
Lincoln, Stephen
;
Srivastava, Ranjan
;
Harris, Steven D.
;
Marten, Mark R.
Número total de Autores: 19
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MBIO; v. 10, n. 2, p. 13-pg., 2019-03-01. |
| Resumo | |
In filamentous fungi, an important kinase responsible for adaptation to changes in available nutrients is cyclic AMP (cAMP)-dependent protein kinase (protein kinase A [PKA]). This kinase has been well characterized at a molecular level, but its systemic action and direct/indirect targets are generally not well understood in filamentous fungi. In this work, we used a pkaA deletion strain (ApkaA) to identify Aspergillus nidulans proteins for which phosphorylation is dependent (either directly or indirectly) on PKA. A combination of phosphoproteomic and transcriptomic analyses revealed both direct and indirect targets of PKA and provided a global perspec- tive on its function. One of these targets was the transcription factor CreA, the main atatton e.) (2!) , &.0 BOPp.. repressor responsible for carbon catabolite repression (CCR). In the ApkaA strain, we identified a previously unreported phosphosite in CreA, 5319, which (based on motif analysis) appears to be a direct target of Stk22 kinase (AN5728). Upon replacement of CreA 5319 with an alanine (i.e., phosphonull mutant), the dynamics of CreA import to the nucleus are affected. Collectively, this work provides a global overview of PKA function while also providing novel insight regarding significance of a specific PKA-mediated phosphorylation event. IMPORTANCE The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling Copyright pathway is well conserved across eukaryotes, and previous work has shown that it plays an important role in regulating development, growth, and virulence in a number of fungi. PKA is activated in response to extracellular nutrients and acts to regulate metabolism and growth. While a number of components in the PKA pathway have been defined in filamentous fungi, current understanding does not provide a global perspective on PKA function. Thus, this work is significant in that it comprehensively identifies proteins and functional pathways regulated by PKA in a model filamentous fungus. This information enhances our understanding of PKA action and may provide information on how to manipulate it for specific purposes. (AU) | |
| Processo FAPESP: | 16/18827-7 - Integrando engenharia de proteínas e biologia sintética para desenvolver novos sistemas de high-throughput selection |
| Beneficiário: | Lucas Ferreira Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/20358-5 - Análise sistêmica do papel da proteína quinase A (PKA) no fungo Trichoderma reesei através de fosfoproteômica |
| Beneficiário: | Liliane Fraga Costa Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |