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Increased Myocardial Retention of Mesenchymal Stem Cells Post-MI by Pre-Conditioning Exercise Training

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Vieira, Stella Souza ; Antonio, Ednei Luiz ; de Melo, Brunno Lemes ; Neves dos Santos, Luis Felipe ; Santana, Eduardo Tadeu ; Feliciano, Regiane ; Navarro Marques, Fabio Luiz ; Faria, Daniele de Paula ; Buchpiguel, Carlos Alberto ; Silva Jr, Jose Antonio ; Ferreira Tucci, Paulo Jose ; Serra, Andrey Jorge
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: STEM CELL REVIEWS AND REPORTS; v. 16, n. 4, p. 12-pg., 2020-04-18.
Resumo

Stem cell (SC) therapy is a promising approach to improve post-myocardial infarction (MI) cardiac remodeling, but the proinflammatory microenvironment may lead to SC loss and, therefore, may have a negative impact on therapy. It appears that exercise training (ET) improves myocardial microenvironment for SC transplantation. Therefore, we tested the effect of ET on post-infarction retention of adipose-derived SCs (ADSCs) and its combined effects on the inflammatory microenvironment. Fischer-344 female rats were randomized to one of the following groups: Sham; sedentary coronary occlusion who did not receive ADSCs (sMI); sedentary coronary occlusion who received ADSCs; exercise coronary occlusion who received ADSCs. Rats were trained nine weeks prior to MI, followed by ADSCs transplantation. The MI led to left ventricle (LV) dilation and dysfunction, myocardial hypertrophy and fibrosis, and increased proinflammatory profile compared to Sham rats. Conversely, ADSCs transplanted rats exhibited, better morphological and functional LV parameters; inhibition of myocardial hypertrophy and fibrosis; and attenuation of proinflammatory cytokines (interleukins 1 beta and 10, tumor necrosis factor alpha, and transforming growth factor beta) in the myocardium compared to sMI rats. Interestingly, ET enhanced the effect of ADSCs on interleukin 10 expression. There was a correlation between cytokine expression and myocardial ADSCs retention. The. ET enhanced the beneficial effects of ADSCs in infarcted myocardium, which was associated with higher ADSCs retention. These findings highlight the importance of ET in myocardial retention of ADSCs and attenuation of cardiac remodeling post-infarction. Cytokine analysis suggests improvement in ET-linked myocardial microenvironment based on its anti-inflammatory action. (AU)

Processo FAPESP: 18/06865-7 - Fotobiomodulação com diodo emissor de luz (LED) pode melhorar a eficiência da terapia com células tronco mesenquimais na insuficiência cardíaca pós-infarto do miocárdio?
Beneficiário:Andrey Jorge Serra
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/54225-8 - Fisiopatologia da insuficiência cardíaca congestiva
Beneficiário:Paulo Jose Ferreira Tucci
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/11028-9 - A modalidade de treinamento físico aeróbio pode determinar a existência de remodelamento cardíaco: identificando peculiaridades estruturais, funcionais e moleculares dos modelos experimentais de natação e corrida
Beneficiário:Andrey Jorge Serra
Modalidade de apoio: Auxílio à Pesquisa - Regular