In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages

Autor(es)

Neris, Debora Meira ; Ortolani, Leticia Goncalves ; Castro, Cynthia Aparecida de ; Correia, Ricardo de Oliveira ; Rodolpho, Joice Margareth de Almeida ; Camillo, Luciana ; Nogueira, Camila Tita ; Sousa, Cristina Paiva de ; Anibal, Fernanda de Freitas

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MICROBIOLOGY; v. 2020, p. 11-pg., 2020-08-27.

Resumo

Leishmaniases are diseases with high epidemiological relevance and wide geographical distribution. In Brazil,Leishmania (Leishmania) amazonensisis related to the tegumentary form of leishmaniasis. The treatment for those diseases is problematic as the available drugs promote adverse effects in patients. Therefore, it is important to find new therapeutic targets. In this regard, one alternative is the study of biomolecules produced by endophytic microorganisms. In this study, the total extract produced by the endophyticPaenibacillus polymyxaRNC-D was used to evaluate the leishmanicidal, nitric oxide, and cytokines production using RAW 264.7 macrophages. The results showed that, in the leishmanicidal assay withL. amazonensis, EC50 values at the periods of 24 and 48 hours were 0.624 mg/mL and 0.547 mg/mL, respectively. Furthermore, the cells treated with the extract presented approximately 25% of infected cells with an average of 3 amastigotes/cell in the periods of 24 and 48 hours. Regarding the production of cytokines in RAW 264.7 macrophages infected/treated with the extract, a significant increase in TNF-alpha was observed at the periods of 24 and 48 hours in the treated cells. The concentrations of IFN-gamma and IL-12 showed significant increase in 48 hours. A significant decrease in IL-4 was observed in all cells treated with the extract in 24 hours. It was observed in the treated cells that the NO production by RAW 264.7 macrophages increased between 48 and 72 hours. The endophyticPaenibacillus polymyxaRNC-D extract modulates the mediators of inflammation produced by RAW 264.7 macrophages promotingL. amazonensisdeath. The immunomodulatory effects might be a promising target to develop new immunotherapeutic and antileishmanial drugs. (AU)

• Processo FAPESP: 16/13423-5 - Compostos produzidos por microrganismos endofíticos isolados de plantas do cerrado: bioatividade, associação com a-Ag2WO4 e aplicações biotecnológicas
• Beneficiário: Cristina Paiva de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/07331-5 - Proteômica e bioprospecção dos efeitos da Mentha piperita L. durante a infecção experimental pelo Schistosoma mansoni
• Beneficiário: Fernanda de Freitas Anibal
• Modalidade de apoio: Auxílio à Pesquisa - Regular