Chemokines and cytokines profile in whole saliva of patients with periodontitis

Autor(es)

Kawamoto, Dione ; Lucas Amado, Pamela Pontes ; Albuquerque-Souza, Emmanuel ; Bueno, Manuela Rocha ; Vale, Glauber Campos ; Saraiva, Luciana ; Alves Mayer, Marcia Pinto

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 135, p. 9-pg., 2020-11-01.

Resumo

Clinical features suggest differences in immune response among periodontitis forms, albeit a large number of cytokines and chemokines remain to be evaluated. The saliva is an available source of mediators and its analysis would be valuable in order to understand pathophysiological differences. The objective of this study was analyze chemokines/cytokines profile in whole saliva of individuals with severe periodontitis (Stage III) presenting moderate [Grade B; GB] or rapid progression rate with a localized incisor-molar pattern [Grade C; GC/IMP]. A case-control study was designed for each periodontitis group. GB (n = 9) and GC/IMP (n = 7) patients and their healthy controls (C-GB, n = 9 and C-GC, n = 7) were evaluated. Non-stimulated saliva samples were assessed by a multiplex assay for a total of 40 cytokines, C-C and C-X-C motif chemokines. GC/IMP group presented higher levels of CCL17 and CCL27 (p = 0.04, FDR > 0.05), and lower levels of CCL2 (p = 0.04, FDR > 0.05) and CCL25 (p = 0.006, FDR < 0.05) when compared to its control. GB patients had higher levels of IL-6, IL-1 beta (p = 0.04, FDR > 0.05), and elevated pro-inflammatory (TNF-alpha,IL-1 beta,INF-gamma,IL-6, IL-16): anti-inflammatory (IL-2, IL-4, IL-10) ratio (p = 0.01, FDR < 0.05) compared to its control [p-values by Mann-Whitney test, and False Discovery Rate (FDR) by Benjamini-Hochburg corrections]. CCL-chemokines and cytokines contributed to differences between GC/C-GC and GB/C-GB, respectively (p < 0.05, PERMANOVA test). These preliminary data revealed that each periodontitis phenotype presented distinct immune profiles differentially expressed in saliva compared to their related controls, suggesting differences in the etiopathogenesis of GB and GC/IMP. (AU)

• Processo FAPESP: 16/13159-6 - Análise da diversidade da microbiota supra e subgengival e perfil de proteínas da saliva e da película do esmalte de indivíduos com periodontite crônica e sucessão microbiana
• Beneficiário: Dione Kawamoto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/16377-7 - Avaliação do efeito de bactérias probióticas sobre a resposta imune contra a.actinomycetemcomitans: estudo em células epiteliais gengivais e em modelo experimental de infecção in vivo.
• Beneficiário: Manuela Rocha Bueno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/00259-0 - Análise da diversidade da microbiota supra e subgengival e perfil de proteínas da saliva e película adquirida de indivíduos com periodontite agressiva
• Beneficiário: Pâmela Pontes Penas Amado
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/10829-3 - Efeito imunomodulatório de bactérias probióticas:análise em monócitos periféricos e células dendríticas.
• Beneficiário: Gláuber Campos Vale
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/18273-9 - Novas estratégias para o controle das periodontites
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Temático