Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by(18)F-FDG PET

Autor(es)

Halpern, Bruno ; Mancini, Marcio C. ; Mendes, Caroline ; Machado, Camila Maria Longo ; Prando, Silvana ; Sapienza, Marcelo Tatit ; Buchpiguel, Carlos Alberto ; do Amaral, Fernanda Gaspar ; Cipolla-Neto, Jose

• Tipo de documento: Artigo Científico
• Fonte: DIABETOLOGY & METABOLIC SYNDROME; v. 12, n. 1, p. 7-pg., 2020-09-21.

Resumo

Objective Melatonin has been shown to increase brown adipose tissue (BAT) mass, which can lead to important metabolic effects, such as bodyweight reduction and glycemic improvement. However, BAT mass can only be measured invasively and. The gold standard for non-invasive measurement of BAT activity is positron emission tomography with 2-deoxy-2-[fluorine-18] fluoro-d-glucose (F-18-FDG PET). There is no study, to our knowledge, that has evaluated if melatonin influences BAT activity, measured by this imaging technique in animals. Methods Three experimental groups of Wistar rats (control, pinealectomy, and pinealectomy replaced with melatonin) had an(18)F-FDG PET performed at room temperature and after acute cold exposure. The ratio of increased BAT activity after cold exposure/room temperature was called "acute thermogenic capacity" (ATC) We also measured UCP-1 mRNA expression to correlate with the(18)F-FDG PET results. Results Pinealectomy led to reduced acute thermogenic capacity, compared with the other groups, as well as reduced UCP1 mRNA expression. Conclusion Melatonin deficiency impairs BAT response when exposed to acute cold exposure. These results can lead to future studies of the influence of melatonin on BAT, in animals and humans, without needing an invasive evaluation of BAT. (AU)

• Processo FAPESP: 14/50457-0 - Melatonina e a regulação do metabolismo energético: estudos básicos, clínicos e epidemiológicos
• Beneficiário: José Cipolla Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático