Cyclophosphamide modulated the foreign body inflammatory reaction in tilapia (Oreochromis niloticus)

Autor(es)

Charlie-Silva, Ives ; Conde, Gabriel ; Mendonca Gomes, Juliana Moreira ; da Rosa Prado, Ed Johnny ; Fernandes, Dayanne Carla ; de Moraes, Alessandra Cristina ; Eto, Silas Fernandes ; Conceicao, Katia ; de Andrade Belo, Marco Antonio

• Tipo de documento: Artigo Científico
• Fonte: FISH & SHELLFISH IMMUNOLOGY; v. 107, p. 8-pg., 2020-12-01.

Resumo

In order to understand events and mechanisms present in the pathophysiology of tilapia's chronic inflammation and based on the immunomodulatory activity attributed to cyclophosphamide which is widely used to suppress immune responses in human medicine, the present study investigated the effects of cyclophosphamide (CYP) treatment on the modulation of foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus) with round glass coverslip implanted in the subcutaneous tissue (9 mm of diameter). Forty tilapia (151 +/- 10,2 g) were randomly distributed in 5 aquariums (n = 8) with a capacity of 250 L of water each, to compose two treatments (sampled 3 and 6 days post-implantation): implanted/untreated (control) and implanted/treated with 200 mg of CYP kg(-1) of b.w., through i.p. route. A fifth group (n = 8) was sampled without any stimulus (naive) to obtain reference values. CYP-treated tilapia showed decrease in macrophage accumulation, giant cell formation and Langhans cells on the glass coverslip when compared to control fish. The treatment with CYP resulted in decrease of leukocyte and thrombocyte counts. Decrease in alpha-2-macroglobulin, ceruloplasmin, albumin and transferrin levels, as well as increase in haptoglobin, complement C3 and apolipoprotein A1 were observed in tilapias during foreign body inflammation. Blood levels of complement C3, alpha-2-macroglobulin, ceruloplasmin and transferrin were modulated by treatment with CYP. Therefore, the treatment with 200 mg of CYP kg(-1) of b.w. in tilapia resulted in an anti-inflammatory effect by suppressing the dynamics between leukocytes in the bloodstream and macrophage accumulation with giant cell formation in the inflamed focus, as well as by modulating APPs during foreign body reaction. (AU)

• Processo FAPESP: 13/11675-9 - Estudo de eficácia terapêutica, segurança clínica e ecotoxicológica do antibiótico Fosfomicina, administrado via oral, para o tratamento de Aeromonas hydrophila e Streptococcus agalactiae em Tilápias do Nilo, Oreochromis niloticus
• Beneficiário: Roberto Barbuio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/12256-7 - Estudo da participação da insulina e do efeito glicocorticóide exógeno sobre a resposta inflamatória aguda de tilápias do Nilo, Oreochromis niloticus, desafiadas com Aeromonas hydrophila.
• Beneficiário: Marco Antonio de Andrade Belo
• Modalidade de apoio: Auxílio à Pesquisa - Regular