Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Estrogen and Insulin Replacement Therapy Modulates the Expression of Insulin-Like Growth Factor-I Receptors in the Salivary Glands of Diabetic Mice

Texto completo
Autor(es):
Yashida, Monica Hayashi [1] ; Domingues Da Silva Faria, Ana Luyza [1] ; Caldeira, Eduardo Jose [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] FMJ, Dept Morphol & Basic Pathol, Tissue Morphol Lab, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 294, n. 11, p. 1930-1938, NOV 2011.
Citações Web of Science: 5
Resumo

Diabetes mellitus results in various complications, also compromising the salivary glands. Hormone levels and interactions with cellular receptors are altered, intensifying the damage caused by this disease. Hormone replacement therapy alone or combined with other treatments may reverse this damage, but doubts still exist regarding the efficacy of this procedure. The objective of this study was to evaluate the effect of estrogen replacement therapy combined with insulin treatment on salivary secretory cells and on the expression of insulin-like growth factor (IGF)-I receptors in salivary glands of spontaneously diabetic (NOD) mice. Twenty-five mice were divided into five groups of five animals each: group I (NOD diabetic), group II (NOD diabetic treated with insulin), group III (NOD diabetic treated with estrogen), group IV (NOD diabetic treated with insulin and estrogen), and group V (control Balb/c mice). Group II received insulin, group III received estrogen, and group IV received insulin plus estrogen administered daily for 20 days. Groups I and V received saline for the same period of time to simulate treatment. Glucose and estrogen levels were monitored during treatment, and salivary gland samples were collected at the end of treatment for stereological analysis and immunofluorescence detection of IGF-I receptors. Tissue restructuring and regulation of IGF-I receptors expression were observed in animals submitted to estrogen replacement therapy plus insulin. Estrogen effectively promoted the recovery of salivary secretory cells, demonstrating that this hormone alone, and especially when combined with insulin, might be important for the reversal of hyperglycemia-induced tissue injury. Anat Rec, 294:1930-1938, 2011. (C) 2011 Wiley-Liss, Inc. (AU)

Processo FAPESP: 08/55521-7 - Efeito da reposição estrogênica e do tratamento insulínico prolongado sobre as glândulas salivares de camundongos espontaneamente diabéticos
Beneficiário:Eduardo José Caldeira
Linha de fomento: Auxílio à Pesquisa - Regular