Integrative network analysis of differentially methylated regions to study the impact of gestational weight gain on maternal metabolism and fetal-neonatal growth

Autor(es)

Perla Pizzi Argentato ^[1] ; João Victor da Silva Guerra ; Liania Alves Luzia ^[3] ; Ester Silveira Ramos ^[4] ; Mariana Maschietto ; Patrícia Helen de Carvalho Rondó ^[6]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Nutrição - Brasil

^[3] Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Nutrição - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética - Brasil

^[6] Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Nutrição - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 47, n. 1 2024-03-25.

Resumo

Abstract Integrative network analysis (INA) is important for identifying gene modules or epigenetically regulated molecular pathways in diseases. This study evaluated the effect of excessive gestational weight gain (EGWG) on INA of differentially methylated regions, maternal metabolism and offspring growth. Brazilian women from “The Araraquara Cohort Study” with adequate pre-pregnancy body mass index were divided into EGWG (n=30) versus adequate gestational weight gain (AGWG, n=45) groups. The methylome analysis was performed on maternal blood using the Illumina MethylationEPIC BeadChip. Fetal-neonatal growth was assessed by ultrasound and anthropometry, respectively. Maternal lipid and glycemic profiles were investigated. Maternal triglycerides-TG (p=0.030) and total cholesterol (p=0.014); fetus occipito-frontal diameter (p=0.005); neonate head circumference-HC (p=0.016) and thoracic perimeter (p=0.020) were greater in the EGWG compared to the AGWG group. Multiple linear regression analysis showed that maternal DNA methylation was associated with maternal TG and fasting insulin, fetal abdominal circumference, and fetal and neonate HC. The DMRs studied were enriched in 142 biological processes, 21 molecular functions,and 17 cellular components with terms directed for the fatty acids metabolism. Three DMGMs were identified:COL3A1, ITGA4 and KLRK1. INA targeted chronic diseases and maternal metabolism contributing to an epigenetic understanding of the involvement of GWG in maternal metabolism and fetal-neonatal growth. (AU)

• Processo FAPESP: 18/17824-0 - Perfil da metilação do DNA do binômio mãe-concepto no contexto da Obesidade materna e adiposidade fetal e neonatal
• Beneficiário: Perla Pizzi Argentato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/03333-6 - Relação entre adiposidade materna e adiposidade do concepto nos períodos fetal, neonatal e no primeiro ano de vida: estudo prospectivo de base populacional
• Beneficiário: Patricia Helen de Carvalho Rondó
• Modalidade de apoio: Auxílio à Pesquisa - Temático