ENAM gene polymorphisms associated with dental anomalies in individuals with cleft lip and palate

Autor(es)

Fernanda Veronese de Oliveira ^[1] ; Carlos Ferreira dos Santos ^[2] ; Thiago José Dionísio ^[3] ; Lucimara Teixeira das Neves ; Gisele da Silva Dalben ^[5] ; Eloá Cristina Passucci Ambrosio ^[6] ; Paula Karine Jorge ^[7] ; Maria Aparecida Andrade Moreira Machado ^[8] ; Thais Marchini Oliveira

Afiliação do(s) autor(es)

^[1] University of São Paulo. Bauru School of Dentistry. Department of Pediatric Dentistry, Orthodontics and Community Health - Brasil

^[2] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[3] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[5] University of São Paulo. Hospital Rehabilitation Anomalies Craniofacial - Brasil

^[6] University of São Paulo. Hospital Rehabilitation Anomalies Craniofacial - Brasil

^[7] University of São Paulo. Hospital Rehabilitation Anomalies Craniofacial - Brasil

^[8] University of São Paulo. Bauru School of Dentistry. Department of Pediatric Dentistry, Orthodontics and Community Health - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Braz. J. Oral Sci.; v. 23, 2024-04-05.

Resumo

Aim This study aimed to investigate the occurrence of enamelin gene (ENAM) single nucleotide polymorphisms (SNP) and ENAM polymorphism association with dental anomalies (DA) in individuals with unilateral or bilateral cleft lip and palate (CLP). Methods Saliva samples were collected from 147 individuals aged between 6 and 15 years-old, both genders, and divided into 4 groups: Group 1 (G1) - CLP and DA; Group 2 (G2) - CLP without DA; Group 3 (G3) - without CLP with DA; Group 4 (G4) - without CLP and DA. The genomic DNA was extracted from saliva samples and the following ENAM SNPs markers were genotyped: rs3796703, rs3796704, rs3796705, rs7671281, rs2609428, and rs35951442. Fisher exact and Pearson’s Chi-square tests statistically analyzed the results (α=5%). Results Individuals without CLP with DA (Group 3 - 19.2%) showed statistically higher prevalence of SNP rs2609428 heterozygotes (p=0.006) than individuals with CLP and DA (Group 1 - 0%). Individuals without CLP (10%) exhibited statistically higher prevalence of mutated heterozygotes/homozygous (p=0.028) than in individuals with CLP (1.3%). Conclusion SNP rs2609428 marker of ENAM gene may be associated with dental anomalies in individuals without cleft lip and palate. (AU)

• Processo FAPESP: 08/08927-8 - Triagem de mutação nos genes IRF6, MSX1 e PAX9 em indivíduos com fissura de lábio e palato e agenesia dentária
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/21190-7 - Triagem de mutações e polimorfismos de genes candidatos à malformação dentária em pacientes com fissura labiopalatina
• Beneficiário: Fernanda Veronese de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/12233-4 - Estudo genético da mutação dos genes AMELX e ENAM na malformação dentária de pacientes com e sem fissura labiopalatina
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular