| Texto completo | |
| Autor(es): |
Flor, Karine C.
;
Maia, Octavio A. C.
;
Takakura, Ana C.
;
Moreira, Thiago S.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY; v. 327, n. 4, p. 12-pg., 2024-10-06. |
| Resumo | |
Considering that the retrotrapezoid nucleus/respiratory parafacial region (RTN/pFRG) would be an important center in the central nervous system involved in the maintenance and modulation of respiratory activity, we hypothesized that neurons in this nucleus would also be involved in the postinspiratory (post-I) phase of the respiratory cycle through a connection with the pontine K & ouml;lliker-Fuse (KF) region. Here, we performed pharmacogenetic manipulation (AAV-hM3D(Gq)-mCherry or AAV-hM4D(Gi)-mCherry) in VGlut2-cre, Ai6 conscious mice to evaluate breathing parameters through whole body plethysmography under baseline conditions (normoxia: FIO2 = 0.21) or under hypercapnia or hypoxia challenges (FICO2 = 0.07 or FIO2 = 0.08). Under normoxia, selective stimulation of RTN/pFRG resulted in a smaller increase in Ve (1,272 +/- 102.5, vs. RTN/pFRG stimulation: 1,878 +/- 122.1 mL/kg/min), due to a smaller increase in VT (5.4 +/- 0.35, vs. RTN/pFRG stimulation: 7.77 +/- 0.21 mL/kg) without changing f(R) in a condition of KF inhibition. However, inhibition of the VGlut2 neurons in the KF did affect the T-E1 produced by selective activation of RTN/pFRG (119.9 +/- 2.53, vs. RTN/pFRG stimulation: 104 +/- 2.46 ms). Both the hypercapnia and hypoxia ventilatory response were reduced after inhibition of VGlut2-expressing KF neurons. Therefore, consistent with anatomical projections RTN/pFRG neurons regulate lung ventilation by controlling all aspects of breathing, i.e., breathing frequency, inspiration, postinspiration, and active expiration. All the modulation seems to be dependent on the integrity of the glutamatergic neurons in the KF region. NEW & NOTEWORTHY Our research reveals specific roles and interactions between the retrotrapezoid nucleus/respiratory parafacial region (RTN/pFRG) and the pontine K & ouml;lliker-Fuse (KF) region in controlling respiratory phases. RTN/pFRG neurons are key in regulating all aspects of breathing, including frequency, inspiration, postinspiration, and active expiration. This regulation depends on the functional integrity of glutamatergic neurons in the KF region, aligning with anatomical projections. (AU) | |
| Processo FAPESP: | 15/18842-3 - Mecanismos orexinérgicos e aminérgicos no controle respiratório em modelo animal de Doença de Parkinson |
| Beneficiário: | Luiz Marcelo Oliveira Santos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/09323-3 - Regulação do fluxo sanguíneo na superfície ventrolateral do bulbo em modelo animal de Doença de Parkinson |
| Beneficiário: | Luiz Marcelo Oliveira Santos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/01236-4 - Efeitos de tratamentos farmacológicos e não farmacológicos nas alterações respiratórias observadas em um modelo murino da Doença de Parkinson |
| Beneficiário: | Ana Carolina Takakura Moreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/20504-0 - Alterações respiratórias promovidas pela estimulação de receptores taquicinérgicos na região do núcleo retrotrapezóide |
| Beneficiário: | Octávio Augusto de Carvalho Maia |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 15/23376-1 - Núcleo retrotrapezóide, quimiossensibilidade central e automaticidade respiratória |
| Beneficiário: | Thiago dos Santos Moreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |