Rho-Kinase Is Differentially Expressed in the Adipose Tissue of Rodent and Human in Response to Aging, Sex, and Acute Exercise

Autor(es):Mostrar menos -

Munoz, Vitor Rosetto ; Vieira, Renan Fudoli Lins ; Katashima, Carlos Kiyoshi ; Gaspar, Rafael Calais ; Lino, Marsel ; Trombeta, Joice Cristina dos Santos ; Duft, Renata Garbellini ; Azevedo Macedo, Ana Paula ; da Silva, Adelino Sanchez Ramos ; Ropelle, Eduardo Rochete ; de Moura, Leandro Pereira ; Cintra, Dennys Esper ; Chacon-Mikahil, Mara Patricia Traina ; Cavaglieri, Claudia Regina ; Pauli, Jose Rodrigo

• Tipo de documento: Artigo Científico
• Fonte: JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES; v. 79, n. 3, p. 10-pg., 2024-02-20.

Resumo

White adipose tissue (WAT) controls energy storage, expenditure, and endocrine function. Rho-kinase (ROCK) is related to impaired thermogenesis, downregulation of preadipocyte differentiation, and adipokine production. Furthermore, WAT ROCK responds to metabolic stress from high-fat diets or diabetes. However, ROCK distribution in adipose depots and its response to aging and sex remain unclear. Thus, we aim to investigate ROCK function in adipose tissue of rodent and human in response to aging and sex. We observed specific differences in the ROCK1/2 distribution in inguinal WAT (ingWAT), perigonadal WAT (pgWAT), and brown adipose tissue of male and female rodents. However, ROCK2 expression was lower in female ingWAT compared with males, a fact that was not observed in the other depots. In the pgWAT and ingWAT of male and female rodents, ROCK activity increased during development. Moreover, middle-aged female rodents and humans showed downregulation in ROCK activity after acute physical exercise. Interestingly, ROCK levels were associated with several inflammatory markers both in rats and humans WAT (Nfkb1, Tnf, Il1b, Il6, and Mcp1). Induction of cell senescence by etoposide elevates ROCK activity in human preadipocytes; however, silencing ROCK1/2 demonstrates improvement in the inflammatory and cell senescence state. Using public databases, several pathways were strongly associated with ROCK modulation in WAT. In summary, WAT ROCK increases with development in association with inflammatory markers. Further, ROCK activity was attenuated by acute physical exercise, implicating it as a possible therapeutic target for metabolism improvement mediated by adipose tissue inflammatory state changes. (AU)

• Processo FAPESP: 20/13443-1 - Implicações do exercício físico aeróbio sobre a via de sinalização da Notch 1 e regulação da lipogênese e gliconeogênese no fígado
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/08692-5 - Análise do interactoma da proteína rev-erb-alfa através da imunoprecipitação da proteína alvo e identificação de possíveis ligantes por espectrometria de massa em cultura de células
• Beneficiário: Vitor Rosetto Muñoz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/20872-6 - Efeitos do exercício físico sobre a via de sinalização da proteína Rock e regulação da lipogênese e gliconeogênese no fígado de ratos envelhecidos
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/18488-8 - O papel do exercício físico nas vias moleculares de captação de glicose em camundongos nocautes para GLUT-4
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/11820-5 - Função do Nr1d1 sobre a Sarcopenia associada ao envelhecimento
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/26000-2 - O papel da proteína Rock sobre a captação de glicose no músculo esquelético de roedores exercitados durante o processo de envelhecimento
• Beneficiário: Vitor Rosetto Muñoz
• Modalidade de apoio: Bolsas no Brasil - Mestrado