Osteopontin, a chemotactic protein with cytokine-like properties, is up-regulated in muscle injury caused by Bothrops lanceolatus (fer-de-lance) snake venom

Autor(es)

Barbosa-Souza, Valeria ^{[1, 2]} ; Contin, Daniel Kiss ^{[1, 2]} ; Bonventi Filho, Waldemar ^[3] ; de Araujo, Albetiza Lobo ^[1] ; Irazusta, Silvia Pierre ^{[3, 2, 4]} ; da Cruz-Hoefling, Maria Alice ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Ciencias Med, Dept Farmacol, UNICAMP, BR-13081970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Histol & Embriol, UNICAMP, BR-13083970 Campinas, SP - Brazil

^[3] CEETPS, Fac Tecnol Sorocaba, BR-18013280 Sorocaba, SP - Brazil

^[4] CEETPS, Programa Posgrad, BR-01124010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 58, n. 5, p. 398-409, OCT 2011.
• Citações Web of Science: 9

Resumo

Osteopontin (OPN) is a chemotactic, adhesive protein whose receptors include some integrins and matrix proteins known to have role in inflammatory and repair processes. We examined the time course of OPN expression at acute and chronic stages after intramuscular injection of Bothrops lanceolatus venom in rats. Additionally, we examined the expression of CD68 (a marker for phagocytic macrophages) and the myogenic factors, myoD and myogenin. There was a biphasic upregulation of OPN (6-48 h and 3-14 days post-venom), i.e., during acute inflammation and myogenic cell proliferation and differentiation phases. OPN was detected in CD68 + macrophages, fibroblasts, normal and damaged myofibers, myoblasts and myotubes. Myogenin was expressed in the cytoplasm (atypical pattern) and nucleus of myoblasts and myotubes from 18 h to 7 days, after which it was expressed only in nuclei. Macrophage numbers, OPN and myogenin expression were still elevated at 7, 14 and 7 days. At 3 days, when OPN achieved the peak, some clusters of myoblasts were within regions of intense collagen deposition. Fibrosis may represent limitation for repairing processes and may explain the small diameter of regenerated fibers at 21 days post-venom. The expression of OPN in the course of venom-induced damage and regeneration suggests stages-specific mediation role along the whole process. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/60929-7 - Lesão do músculo esquelético de ratos induzida pelo veneno da Bothrops lanceolatus
• Beneficiário: Albetiza Lôbo de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular