| Texto completo | |
| Autor(es): |
Martins, Isabela Mancini
;
Seribelli, Amanda Aparecida
;
Ribeiro, Tamara R. Machado
;
da Silva, Patrick
;
Lustri, Bruna Cardinali
;
Hernandes, Rodrigo T.
;
Falcao, Juliana Pfrimer
;
Moreira, Cristiano Gallina
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INFECTION GENETICS AND EVOLUTION; v. 116, p. 10-pg., 2023-11-16. |
| Resumo | |
Invasive non-typhoidal Salmonella (iNTS) from the clonal type ST313 (S. Typhimurium ST313) is the primary cause of invasive salmonellosis in Africa. Recently, in Brazil, iNTS ST313 strains have been isolated from different sources, but there is a lack of understanding of the mechanisms behind how these gut bacteria can break the gut barrier and reach the patient's bloodstream. Here, we compare 13 strains of S. Typhimurium ST313, previously unreported isolates, from human blood cultures, investigating aspects of virulence and mechanisms of resistance. Initially, RNAseq analyses between ST13-blood isolate and SL1344 (ST19) prototype revealed 15 upregulated genes directly related to cellular invasion and replication, such as sopD2, sifB, and pipB. Limited information is available about S. Typhimurium ST313 pathogenesis and epidemiology, especially related to the global distribution of strains. Herein, the correlation of strains isolated from different sources in Brazil was employed to compare clinical and non-clinical isolates, a total of 22 genomes were studied by single nucleotide polymorphism (SNPs). The epidemiological analysis of 22 genomes of S. Typhimurium ST313 strains grouped them into three distinct clusters (A, B, and C) by SNP analysis, where cluster A comprised five, group B six, and group C 11. The 13 clinical blood isolates were all resistant to streptomycin, 92.3% of strains were resistant to ampicillin and 15.39% were resistant to kanamycin. The resistance genes acrA, acrB, mdtK, emrB, emrR, mdsA, and mdsB related to the production of efflux pumps were detected in all (100%) strains studied, similar to pathogenic traits investigated. In conclusion, we evidenced that S. Typhimurium ST313 strains isolated in Brazil have unique epidemiology. The elevated frequencies of virulence genes such as sseJ, sopD2, and pipB are a major concern in these Brazilian isolates, showing a higher pathogenic potential. (AU) | |
| Processo FAPESP: | 22/05030-4 - Estudos de heteroresistência em patógenos emergentes no Brasil |
| Beneficiário: | CRISTIANO GALLINA MOREIRA |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |
| Processo FAPESP: | 19/19338-8 - Genômica, transcriptômica e caracterização fenotipica de linhagens de Campylobacter jejuni isoladas de diversas fontes durante 20 anos no Brasil |
| Beneficiário: | Juliana Pfrimer Falcão |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/06633-6 - Análise comparativa do genoma, transcriptoma e caracterização fenotípica de linhagens de Salmonella typhimurium isoladas de humanos e alimentos no Brasil |
| Beneficiário: | Amanda Aparecida Seribelli |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/03049-7 - Transcriptoma da sinalização química bacteriana em Salmonella Typhimurium, investigação de enteropatógenos bacterianos globais emergentes em São Paulo e emprego de modelo alternativo de infecção no estudo da patogênese. |
| Beneficiário: | CRISTIANO GALLINA MOREIRA |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/26696-8 - Estudo de biomoduladores microencapsulados com atividades antimicrobiana e antivirulência em cepas de Escherichia coli uropatogênica multi-droga resistentes |
| Beneficiário: | Bruna Cardinali Lustri |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |