Fish Oil Supplementation Mitigates High-Fat Diet-Induced Obesity: Exploring Epigenetic Modulation and Genes Associated with Adipose Tissue Dysfunction in Mice

Autor(es)

Simao, Jussara de Jesus ; Bispo, Andressa Franca de Sousa ; Plata, Victor Tadeu Goncalves ; Armelin-Correa, Lucia Maria ; Alonso-Vale, Maria Isabel Cardoso

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICALS; v. 17, n. 7, p. 19-pg., 2024-07-01.

Resumo

This study investigated the effects of fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA), on obesity induced by a high-fat diet (HFD) in mice. The investigation focused on elucidating the impact of FO on epigenetic modifications in white adipose tissue (WAT) and the involvement of adipose-derived stem cells (ASCs). C57BL/6j mice were divided into two groups: control diet and HFD for 16 weeks. In the last 8 weeks, the HFD group was subdivided into HFD and HFD + FO (treated with FO). WAT was removed for RNA and protein extraction, while ASCs were isolated, cultured, and treated with leptin. All samples were analyzed using functional genomics tools, including PCR-array, RT-PCR, and Western Blot assays. Mice receiving an HFD displayed increased body mass, fat accumulation, and altered gene expression associated with WAT inflammation and dysfunction. FO supplementation attenuated these effects, a potential protective role against HFD-induced obesity. Analysis of H3K27 revealed HFD-induced changes in histone, which were partially reversed by FO treatment. This study further explored leptin signaling in ASCs, suggesting a potential mechanism for ASC dysfunction in the obesity-rich leptin environment of WAT. Overall, FO supplementation demonstrated efficacy in mitigating HFD-induced obesity, influencing epigenetic and molecular pathways, and shedding light on the role of ASCs and leptin signaling in WAT dysfunction associated with obesity. (AU)

• Processo FAPESP: 19/13618-9 - Estudo da modulação da expressão de Ezh2 e de outros modificadores de H3K27 por fatores da transcrição NF-kB em células adiposas
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/15127-5 - Modulação da via de NF-kB e repercussões sobre marcas epigenéticas em células adiposas: papel do Oncotherad®
• Beneficiário: Andressa Franca de Sousa Bispo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 19/26240-4 - Vias de sinalização afetadas pelo óleo de peixe em células tronco derivadas do tecido adiposo (AdSCs) e adipócitos: correlação com alterações metabólicas, endócrinas e na adipogênese que repercutem em seus efeitos anti-Obesidade Hipertrófica
• Beneficiário: Jussara de Jesus Simão
• Modalidade de apoio: Bolsas no Brasil - Doutorado