| Texto completo | |
| Autor(es): |
Simao, Jussara de Jesus
;
Bispo, Andressa Franca de Sousa
;
Plata, Victor Tadeu Goncalves
;
Armelin-Correa, Lucia Maria
;
Alonso-Vale, Maria Isabel Cardoso
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHARMACEUTICALS; v. 17, n. 7, p. 19-pg., 2024-07-01. |
| Resumo | |
This study investigated the effects of fish oil (FO) treatment, particularly enriched with eicosapentaenoic acid (EPA), on obesity induced by a high-fat diet (HFD) in mice. The investigation focused on elucidating the impact of FO on epigenetic modifications in white adipose tissue (WAT) and the involvement of adipose-derived stem cells (ASCs). C57BL/6j mice were divided into two groups: control diet and HFD for 16 weeks. In the last 8 weeks, the HFD group was subdivided into HFD and HFD + FO (treated with FO). WAT was removed for RNA and protein extraction, while ASCs were isolated, cultured, and treated with leptin. All samples were analyzed using functional genomics tools, including PCR-array, RT-PCR, and Western Blot assays. Mice receiving an HFD displayed increased body mass, fat accumulation, and altered gene expression associated with WAT inflammation and dysfunction. FO supplementation attenuated these effects, a potential protective role against HFD-induced obesity. Analysis of H3K27 revealed HFD-induced changes in histone, which were partially reversed by FO treatment. This study further explored leptin signaling in ASCs, suggesting a potential mechanism for ASC dysfunction in the obesity-rich leptin environment of WAT. Overall, FO supplementation demonstrated efficacy in mitigating HFD-induced obesity, influencing epigenetic and molecular pathways, and shedding light on the role of ASCs and leptin signaling in WAT dysfunction associated with obesity. (AU) | |
| Processo FAPESP: | 19/13618-9 - Estudo da modulação da expressão de Ezh2 e de outros modificadores de H3K27 por fatores da transcrição NF-kB em células adiposas |
| Beneficiário: | Maria Isabel Cardoso Alonso-Vale |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/15127-5 - Modulação da via de NF-kB e repercussões sobre marcas epigenéticas em células adiposas: papel do Oncotherad® |
| Beneficiário: | Andressa Franca de Sousa Bispo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 19/26240-4 - Vias de sinalização afetadas pelo óleo de peixe em células tronco derivadas do tecido adiposo (AdSCs) e adipócitos: correlação com alterações metabólicas, endócrinas e na adipogênese que repercutem em seus efeitos anti-Obesidade Hipertrófica |
| Beneficiário: | Jussara de Jesus Simão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |