Diversity of Staphylococcus aureus enterotoxin genes and its potential impact on severity of mastitis in dairy cows

Autor(es):Mostrar menos -

Dantas, Stefani T. A. ; Silva, Laura B. B. ; Takume, Laura T. S. ; Rossi, Bruna F. ; Bonsaglia, Erika C. R. ; Junior, Ary Fernandes ; Pantoja, Jose C. F. ; dos Santos, Marcos V. ; Goncalves, Juliano L. ; Ribon, Andrea O. B. ; Silva, Nathalia C. C. ; Rall, Vera L. M.

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 198, p. 6-pg., 2025-01-01.

Resumo

Staphylococcus aureus enterotoxins (SEs) can lead to food poisoning and appear to play a pivotal role in the development and severity of mastitis. This study aimed to evaluate whether the presence and diversity of S. aureus Enterotoxin genes mastitis-causing are associated with the development and severity of mastitis in dairy cows. Comparative analysis of S. aureus isolates from cows with clinical and subclinical mastitis (103 isolates each, CM and SM respectively) was conducted, examining the occurrence of enterotoxins (sea-see, seg-sez, sel26, sel27, sel01 and se02). Clinical isolates exhibited greater diversity, with 14 genes compared to four in subclinical isolates, and higher gene frequency (100 % vs. 86.4 %). Among the classical SEs, only sec (1 %) and sed (4.9 %) were detected in clinical isolates, suggesting a potential insignificance in the development of mastitis. Conversely, the selw and selx genes were among the most prevalent in both clinical and subclinical mastitis isolates. While their role in human food poisoning has not been definitively established, they appear to influence mastitis development. Statistical analysis revealed significant differences in clinical isolates carrying seg, seh, sei, seo, seul1, seul2, selw, and selx genes compared to subclinical isolates, suggesting their potential involvement in the severity of mastitis. Determining the frequency of SE gene and its role in mastitis establishment and in clinical mastitis severity, as well as understanding how enterotoxins contribute to pathogenicity in clinical and subclinical mastitis, is crucial for guiding optimal medical therapy for animals and establishing effective veterinary treatments to mitigate economic losses in the dairy industry. (AU)

• Processo FAPESP: 15/19688-8 - E. coli, Klebsiella pneumoniae e Enterococcus spp: impacto dos fatores de virulência na mastite bovina e reflexos na saúde pública
• Beneficiário: Helio Langoni
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/13775-0 - Fatores de virulência e resposta inflamatória de S. aureus isolados de Mastite clínica e subclínica e expressão de citocinas em células epiteliais mamárias bovinas (BMEC)
• Beneficiário: Erika Carolina Romão Bonsaglia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/09902-3 - Perfil genético de virulência de Escherichia coli e Klebsiella pneumoniae obtidos de vacas com mastite clínica e expressão de citocinas em células epiteliais infectadas.
• Beneficiário: Stéfani Thais Alves Dantas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado